Cyanine Labeling Compound With PEG Linker for High Quantum Yield
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Solution Overview
Problem
Cyanine dyes used for fluorescence labeling exhibit low binding properties to biological molecules, leading to insufficient fluorescence intensity, and self-association interactions result in decreased quantum yield.
Innovation Solution
A compound represented by General Formula (1) with specific structural features, including polymethine chains, PEG groups, and nitrogen-containing rings, which enhance binding to biological substances and suppress self-association, ensuring high fluorescence intensity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Illumination intensity
If cyanine dyes are used for fluorescence labeling, then fluorescence labeling can be performed, but self-association between dyes occurs and fluorescence quantum yield decreases
Solution Approach 1:
A PEG-based linker acts as an intermediary between the cyanine dye and the biological molecule. This linker suppresses self-association of the cyanine dye while maintaining binding to the biological molecule, thereby preserving fluorescence quantum yield and enabling high fluorescence intensity labeling.
2Reliability
If cyanine dyes with PEG groups are used to suppress self-association, then fluorescence quantum yield improves, but binding property to biological molecules decreases
Solution Approach 1:
The fluorescent labeling compound is segmented into distinct functional modules: a cyanine dye moiety for fluorescence emission, a PEG-based linker for suppressing self-association and providing flexibility, and a biological molecule-binding moiety for specific binding. This segmentation allows each module to optimize its specific function without compromising the others.
Solution Approach 2:
The invention creates a composite fluorescent labeling compound combining cyanine dye, PEG linker, and biological molecule-binding groups. This composite structure integrates the fluorescence properties of cyanine dye with the self-association suppression of PEG and the binding capability for biological molecules, achieving both high fluorescence quantum yield and strong binding property.
3Illumination intensity
If conventional cyanine dyes are used, then labeling can be achieved, but sufficient fluorescence intensity cannot be obtained due to low binding property
Solution Approach 1:
The invention creates a composite fluorescent labeling compound combining cyanine dye, PEG linker, and biological molecule-binding groups. This composite structure integrates the fluorescence properties of cyanine dye with the self-association suppression of PEG and the binding capability for biological molecules, achieving both high fluorescence quantum yield and strong binding property.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The compound achieves excellent fluorescence intensity and binding properties, particularly in near-infrared fluorescence labeling, improving sensitivity and reducing interference in multicolor Western blotting and bioimaging applications.
Implementation Method 1
a cyanine dye is known as a fluorescent dye that is used for fluorescence labeling
Implementation Method 2
the absorption and emission waveforms of a plurality of dyes
Data Source
AI summary
A compound of Formula (1) and a labeled biological substance having the compound.Z1 and Z2 represent a specific 6-membered ring, and at least one of Z1 or Z2 is a benzene ring having a specific substituent at an ortho position with respect to a nitrogen atom to which L1 or L2 is bonded, or a specific nitrogen-containing 6-membered ring in which a ring-constituting atom located at the ortho position is a nitrogen atom. The compound has at least one structure represented by —(CH2—CH2—O)m—R21 on a heterocyclic ring, and has at least one substituent capable of being bonded to a carboxy group or a biological substance at a specific position, and in a case where at least one of Z1 or Z2 is the specific nitrogen-containing 6-membered ring, the specific substituents may be bonded to each other to form a ring.