Positively Charged Cyclodextrin Preservation for Ischemia-Reperfusion Injury
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Solution Overview
Problem
Current preservation solutions for organs, tissues, and cells are inadequate in maintaining viability and function during and after transplantation, particularly due to issues with ischemia-reperfusion damage and time constraints, and there is a need for improved solutions to extend preservation time and enhance post-transplant recovery.
Innovation Solution
A composition comprising positively charged modified cyclodextrin, preferably associated with an antioxidant like resveratrol, which is effective in protecting and preserving cells, tissues, or organs by reducing damage from ischemia-reperfusion and improving long-term viability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Duration of action of stationary object
If conventional preservation solutions (UW, IGL-1, Celsior, Custodiol) are used, then organs can be preserved for a limited period, but tissue damage occurs during cold and hot ischemia and worsens at reperfusion
Solution Approach 1:
The patent introduces positively charged cyclodextrins as intermediary molecules that mediate between the preservation solution and organ tissues. These cyclodextrins form protective complexes with membrane components and antioxidants, acting as a buffer against ischemia-reperfusion injury during the preservation period
Solution Approach 2:
The invention creates a composite preservation solution combining positively charged cyclodextrins with antioxidants (such as resveratrol, vitamin C, vitamin E) and other preservation agents. This composite formulation synergistically addresses multiple damage mechanisms simultaneously, improving both preservation duration and tissue protection
2Duration of action of stationary object
If preservation time is extended for marginal donors, then more organs become available for transplantation, but tissue quality and long-term viability deteriorate
Solution Approach 1:
The patent applies prior cushioning by incorporating antioxidants and positively charged cyclodextrins into the preservation solution before the organ is placed. These agents pre-establish protective mechanisms against oxidative stress and membrane damage that will occur during extended preservation, cushioning the organ against future injury
Solution Approach 2:
The invention changes the chemical parameters of the preservation solution by using positively charged cyclodextrins instead of conventional neutral or negatively charged surfactants. This parameter change allows for extended preservation times while maintaining membrane integrity and cellular function through electrostatic interactions with tissue components
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The composition significantly enhances the preservation and functional recovery of organs post-transplantation by improving diuresis, reducing creatinine levels, controlling proteinuria, and minimizing tissue damage, as demonstrated in renal transplantation models.
Implementation Method 1
comprising at least one positively charged modified cyclodextrin
Implementation Method 2
the positively charged modified cyclodextrin is intimately associated with an antioxidant
Data Source
AI summary
The invention relates to a composition for protecting and/or preserving cells, tissues and organs, characterised in that it includes at least one modified positively-charged cyclodextrin preferably closely associated with an anti-oxidant. The invention also relates to a method for obtaining this composition and to the uses thereof.