Cyclodextrin Complexes for Stable Membrane-Bound Wnt and Hh Proteins
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Solution Overview
Problem
Current methods for obtaining membrane-bound Wingless (Wnt) and Hedgehog (Hh) proteins are not effective in yielding stable and efficacious molecules, as they often result in protein denaturation and limited stability.
Innovation Solution
A complex of lipid-modified proteins, such as Wnt and Hh, is formed with cyclodextrin, stabilized by trehalose, and preserved through lyophilization, allowing long-term storage and stability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stability of the object's composition
If current methods are used to obtain membrane-bound Wnt and Hh proteins, then the proteins can be extracted, but they undergo denaturation and have limited stability
Solution Approach 1:
The patent introduces cyclodextrin as an intermediary molecule that forms inclusion complexes with membrane-bound Wnt and Hh proteins. The cyclodextrin acts as a mediator that protects the proteins from denaturation while maintaining their biological activity, thereby resolving the contradiction between protein stability and efficacy
Solution Approach 2:
The patent creates a composite structure by combining cyclodextrin with lipid-modified Wnt and Hh proteins to form stable inclusion complexes. This composite material approach allows the proteins to maintain their membrane-bound characteristics while gaining enhanced stability and reliability for therapeutic applications
2Duration of action of stationary object
If membrane-bound proteins are extracted using conventional methods, then the proteins can be obtained, but they lack long-term storage stability
Solution Approach 1:
The patent changes the physical and chemical parameters of the protein preparation by forming cyclodextrin inclusion complexes. This parameter change enables long-term storage stability while maintaining protein activity, allowing the proteins to be stored without immediate loss of function
Solution Approach 2:
The patent performs preliminary complex formation with cyclodextrin during the protein extraction process. This preliminary action stabilizes the proteins early in the purification process, preventing denaturation during subsequent steps and enabling long-term storage
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method ensures the capture and preservation of lipid-modified proteins, enabling their use in tissue repair, wound healing, skin rejuvenation, and hair growth, with enhanced stability and efficacy.
Implementation Method 1
compositions comprising a complex of lipid-modified proteins and a cyclodextrin
Implementation Method 2
lipid-modified proteins comprise one or more Wingless (Wnt) or Hedgehog (Hh) proteins associated with a cell membrane lipid
Implementation Method 3
the composition further comprises at least one kosmotrope. In some embodiments, the at least one kosmotrope is propylene glycol, proline, trehalose, ectoine, or trimethylamine N-oxide
Implementation Method 4
preserved through lyophilization, allowing long-term storage and stability
Data Source
AI summary
Disclosed herein are compositions comprising complexes of cyclodextrins and lipid-modified stem cell proteins. Also disclosed are topical compositions the complexes. Methods of using the compositions for the therapeutic purposes are also disclosed as well as methods of producing the compositions.