d60 Gene Suppression for Lodging Resistance in Rice
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Solution Overview
Problem
Current short culm genes for gramineous plants, such as the sd1 gene, often reduce panicle and grain size alongside plant height, limiting their use in breeding for lodging resistance and productivity.
Innovation Solution
Identification and utilization of the d60 gene, a functionally-defective form of the Os02g0280200 gene, to suppress its expression in gramineous plants using antisense methods or mutagenesis, allowing for the production of short-culmed plants with normal panicle and grain size but reduced height.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Strength
If the sd1 gene is used to shorten plant height, then lodging resistance is improved, but panicle and grain size are reduced
Solution Approach 1:
The patent changes the genetic parameter from sd1 to d60, which affects different physiological pathways. The d60 gene (a defective form of Os02g0280200) shortens culm length through a mechanism independent of the gibberellin biosynthesis pathway affected by sd1, thereby achieving lodging resistance without the penalty of reduced panicle and grain size
Solution Approach 2:
The patent creates a functional copy of the shortening effect without copying the harmful side effects. By using d60 instead of sd1, the patent obtains a genetic variant that copies the useful function (height reduction for lodging resistance) while avoiding the harmful effect (reduced panicle and grain size) associated with sd1
2Strength
If only one specific gene (sd1) is used for short culm breeding, then lodging resistance is achieved, but genetic diversity is reduced
Solution Approach 1:
The patent segments the short culm gene pool into multiple independent options (sd1 and d60). By identifying d60 as a distinct functional gene on the second chromosome independent from sd1 (on the first chromosome), the patent creates separate genetic pathways for achieving the same phenotypic result, thereby maintaining genetic diversity while preserving lodging resistance
Solution Approach 2:
The patent makes the gene pool universal by demonstrating that multiple genes (sd1 and d60) can independently provide the same useful function (lodging resistance through height reduction). This multi-functionality allows breeders to choose different genetic bases depending on their specific needs, enhancing overall adaptability and genetic diversity
Data Source
AI summary
The purpose of the invention is to identify a short-culm gene other than the sd1 gene and produce a short-culmed gramineous plant by utilizing a short-culm gene other than the sd1 gene. The present invention provides a method for producing a short-culmed gramineous plant utilizing gene d60 for shortening the culm of a gramineous plant, wherein the method for producing a short-culmed gramineous plant is characterized in that the expression of Os02g0280200 is suppressed. The present invention also provides a short-culmed gramineous plant and the like in which the expression of Os02g0280200 is suppressed.