Data-Encoded Nucleic Acid Synthesis via Template-Dependent Polymerase
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Solution Overview
Problem
Current electronic information storage technologies based on silicon face scaling limits and resource depletion, necessitating the development of more compact, renewable data storage solutions using nucleic acids.
Innovation Solution
A method involving polymerase extension reactions and monomer removal processes to produce data-encoded nucleic acids, utilizing modified nucleotides and template-dependent synthesis to encode information efficiently.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If silicon-based memory chips are used for data storage, then data storage capacity and speed are improved, but resource depletion occurs and scaling is limited to about 10 nm
Solution Approach 1:
The patent changes the fundamental material parameter from silicon to nucleic acids, enabling renewable data storage while maintaining high storage capacity. Nucleic acids can be produced from renewable biological sources and offer virtually unlimited scalability without the resource depletion issues of silicon.
Solution Approach 2:
The patent uses template-dependent synthesis where a template nucleic acid guides the copying of data information onto new nucleic acid strands. This allows for renewable production of data-encoded nucleic acids through biological copying mechanisms rather than depleting silicon resources.
2Quantity of substance
If conventional nucleic acid synthesis methods are used, then data-encoded nucleic acids can be produced, but synthesis cost and time are excessively high
Solution Approach 1:
The patent implements continuous template-dependent synthesis reactions where nucleotides are continuously added to extending primer strands guided by the template. This continuous process eliminates repeated purification and setup steps, dramatically reducing both synthesis time and cost while enabling large-scale production.
Solution Approach 2:
The patent performs preliminary annealing of primers to templates before the extension reaction, ensuring proper binding occurs first. This preliminary action prevents mispairing during extension and eliminates the need for multiple correction cycles, reducing overall synthesis time and improving efficiency.
3Manufacturing precision
If conventional nucleic acid synthesis methods are used, then data-encoded nucleic acids can be produced, but manufacturing complexity and cost are excessively high
Solution Approach 1:
The patent employs template-dependent synthesis where the template nucleic acid itself guides the accurate incorporation of complementary nucleotides. The template serves as both the data source and the accuracy guarantee, eliminating the need for complex external verification systems while maintaining high sequence accuracy through natural base-pairing rules.
Solution Approach 2:
The patent uses a universal template-dependent synthesis approach that can produce any data-encoded nucleic acid sequence by simply changing the template input. This single method handles diverse data encoding requirements without requiring different synthesis protocols, reducing manufacturing complexity while maintaining precision.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables efficient production of data-encoded nucleic acids that can store information in a compact form, reducing synthesis costs and time while allowing large-scale production.
Implementation Method 1
an extension reaction, which is a polymerase extension of a data-encoded template DNA
Data Source
AI summary
Provided are a method for producing a data-encoded nucleic acid by using a template-dependent nucleic acid polymerase, and a data-encoded nucleic acid produced thereby.