DBN8002 Soybean Event Detection Using Junction-Specific DNA Sequences
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Solution Overview
Problem
Existing methods for detecting specific transgenic soybean events like DBN8002 are inadequate, particularly in distinguishing between events produced by the same DNA construct, and there is a need for accurate and rapid detection methods to ensure reliable gene expression and compliance with regulatory requirements.
Innovation Solution
The use of specific nucleic acid sequences, including SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, and SEQ ID NO: 4, or their complementary sequences, to design primers and probes for PCR and hybridization methods, allowing precise identification of the transgenic soybean event DBN8002 by targeting the insertion junctions and flanking genomic DNA.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional detection methods are used to identify transgenic soybean events, then detection can be performed, but the ability to distinguish between events produced by the same DNA construct is insufficient
Solution Approach 1:
The patent segments the detection approach by developing event-specific detection methods that target unique characteristics of each transgenic event (DBN8002, DBN8003, DBN8004) rather than using a generic detection method. This segmentation allows precise distinction between events produced by the same DNA construct, directly resolving the technical contradiction by improving detection precision without requiring overly complex integrated systems.
Solution Approach 2:
The patent applies local quality by designing detection methods that target specific local regions or characteristics unique to each transgenic event. By focusing detection on event-specific markers rather than general transgenic markers, the method achieves high precision in distinguishing between similar events while keeping the overall detection framework relatively simple.
2Reliability
If multiple transgenic events are screened to identify optimal gene expression, then reliable gene expression can be achieved, but the time and resources required increase significantly
Solution Approach 1:
The patent implements preliminary action by developing and applying specific detection methods early in the screening process to identify events with optimal gene expression characteristics. By using event-specific detection markers from the beginning, researchers can quickly eliminate unsuitable events without conducting extensive phenotypic screening, thereby reducing the time and resources required while maintaining reliability in identifying optimal events.
Solution Approach 2:
The patent replaces mechanical/phenotypic screening methods with molecular detection methods based on nucleic acid hybridization and PCR. This substitution allows for rapid, high-throughput screening of multiple transgenic events simultaneously, dramatically reducing the time required to identify events with reliable gene expression while maintaining or improving the accuracy of selection.
3Productivity
If transgenic events are identified without event-specific detection methods, then general transgene presence can be detected, but accurate identification of specific events like DBN8002 cannot be achieved
Solution Approach 1:
The patent applies dynamics by creating a flexible detection system that can be adapted to detect different transgenic events by changing the specific probe or primer sequences. This dynamic approach allows the same basic detection framework to rapidly switch between detecting different events (DBN8002, DBN8003, DBN8004) without requiring completely different methodologies, thereby maintaining high detection speed while achieving event-specific accuracy.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables accurate and rapid detection of the transgenic soybean event DBN8002, ensuring reliable gene expression and compliance with regulatory standards through precise identification of the event in biological samples.
Implementation Method 1
The use of specific nucleic acid sequences, including SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, and SEQ ID NO: 4, or their complementary sequences, to design primers and probes for PCR and hybridization methods
Implementation Method 2
The use of specific nucleic acid sequences, including SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, and SEQ ID NO: 4, or their complementary sequences, to design primers and probes for PCR and hybridization methods
Data Source
AI summary
The present invention relates to nucleic acid sequences for detecting soybean plant DBN8002 and detection methods thereof, wherein said nucleic acid sequences comprise SEQ ID NO: 1 or a complementary sequence thereof, and/or SEQ ID NO: 2 or a complementary sequence thereof. The soybean plant DBN8002 of the present invention has good resistance against Lepidoptera insects as well as good tolerance to glufosinate herbicide without compromising the yield, and the detection methods can accurately and rapidly identify whether a biological sample contains the DNA molecule of the transgenic soybean event DBN8002.