DBN8205 Soybean Event Detection via Insertion Junction Sequences
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Solution Overview
Problem
Existing methods for detecting specific transgenic soybean events, such as DBN8205, are inadequate as they often rely on general genetic elements and fail to distinguish between events with similar DNA constructs, necessitating extensive screening and regulatory compliance challenges.
Innovation Solution
The use of specific nucleic acid sequences, including SEQ ID NOs: 1, 2, 3, and 4, which span the insertion junctions and flanking genomic regions, allows for accurate detection of the transgenic soybean event DBN8205 through PCR amplification or hybridization, ensuring precise identification and regulatory compliance.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If general genetic elements are used for detection, then detection coverage is broad, but detection precision is insufficient to distinguish between events with similar DNA constructs
Solution Approach 1:
The patent segments the detection approach by using specific nucleic acid sequences (SEQ ID NOs: 1, 2, 3, and 4) that target unique regions at the insertion junctions and flanking genomic regions of the DBN8205 event. This segmentation allows differentiation from other events with similar DNA constructs while maintaining a relatively simple PCR-based detection framework.
2Measurement precision
If extensive screening is performed to ensure regulatory compliance, then detection accuracy is improved, but time consumption increases
Solution Approach 1:
The patent performs preliminary action by designing specific nucleic acid sequences that span the insertion junctions and flanking genomic regions before detection is needed. These pre-designed primers and probes (SEQ ID NOs: 1, 2, 3, and 4) enable direct and accurate detection of the DBN8205 event without requiring extensive screening procedures, thereby reducing time consumption while maintaining high detection accuracy.
3Measurement precision
If specific nucleic acid sequences spanning insertion junctions are used, then detection precision is improved, but sequence complexity increases
Solution Approach 1:
The patent applies local quality by focusing the detection specificity on local regions - the insertion junctions and flanking genomic regions - rather than requiring complex analysis of the entire transgenic construct. The specific nucleic acid sequences (SEQ ID NOs: 1, 2, 3, and 4) are designed to target these localized unique regions, achieving high event identification precision while keeping the overall sequence complexity manageable.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The proposed nucleic acid sequences enable rapid and accurate detection of DBN8205, facilitating regulatory approval and ensuring consistent expression of insect resistance and herbicide tolerance traits in soybean progeny.
Implementation Method 1
The use of specific nucleic acid sequences, including SEQ ID NOs: 1, 2, 3, and 4, which span the insertion junctions and flanking genomic regions, allows for accurate detection of the transgenic soybean event DBN8205 through PCR amplification or hybridization
Implementation Method 2
The use of specific nucleic acid sequences, including SEQ ID NOs: 1, 2, 3, and 4, which span the insertion junctions and flanking genomic regions, allows for accurate detection of the transgenic soybean event DBN8205 through PCR amplification or hybridization
Data Source
AI summary
Provided are a nucleic acid sequence for detecting a Glycine max plant DBN8205 and a detection method therefor. The nucleic acid sequence comprises SEQ ID NO: 1 or a complementary sequence thereof, and/or SEQ ID NO: 2 or a complementary sequence thereof. The Glycine max plant DBN8205 has good resistance to insects of Lepidoptera order and good tolerance to glufosinate herbicides, and therefore the yield is not affected. By utilizing the detection method, it is possible to accurately and quickly identify whether a biological sample contains a DNA molecule of a transgenic Glycine max event DBN8205.