dCAPS Marker and Primers for Wheat Fusarium Crown Rot Resistance
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Solution Overview
Problem
The lack of effective molecular markers for identifying resistance to Fusarium crown rot (FCR) in wheat, coupled with inconsistent inoculation methods and evaluation criteria, leads to unstable and time-consuming identification processes, hindering the development of disease-resistant wheat varieties.
Innovation Solution
Development of a derived cleaved amplified polymorphic sequence (dCAPS) molecular marker located on wheat chromosome 1B, utilizing a specific primer set for PCR amplification and electrophoresis to identify resistance to Fusarium pseudograminearum, allowing for rapid and stable genotype detection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional phenotypic identification methods are used to screen wheat resistance to FCR, then the identification process can be performed without specialized equipment, but the identification time is extended to months and the results are unstable due to inconsistent inoculation methods and evaluation criteria
Solution Approach 1:
The patent replaces the mechanical/biological phenotypic identification system with a molecular detection system. Specifically, it uses PCR amplification followed by restriction enzyme digestion and electrophoresis to detect the FCR-1B-d8 molecular marker, substituting the complex, time-consuming phenotypic screening process with a standardized molecular biology workflow that delivers results in days rather than months with higher precision and stability.
2Productivity
If molecular marker-assisted selection is implemented to accelerate breeding, then breeding efficiency is improved and identification time is reduced, but the complexity of the detection system and required equipment increases
Solution Approach 1:
The patent segments the detection process into distinct, standardized molecular biology steps: DNA extraction, PCR amplification with specific primers, restriction enzyme digestion, and electrophoresis. Each step uses well-established, commercially available reagents and protocols, making the complex molecular detection system modular and manageable while maintaining high breeding efficiency.
Solution Approach 2:
The patent introduces a specific molecular marker (FCR-1B-d8) as an intermediary that links the complex genetic resistance traits to a simple, detectable molecular signal. This marker serves as a mediator between the complex resistance genes and the straightforward PCR-RFLP detection method, enabling efficient screening without directly assessing complex phenotypic traits.
3Ease of manufacture
If phenotypic identification methods are used, then no specialized molecular equipment is required, but the evaluation criteria are inconsistent and lead to unstable identification results
Solution Approach 1:
The patent changes the detection parameter from variable phenotypic traits (which depend on inoculation conditions, growth environment, and subjective evaluation) to a fixed molecular parameter (presence or absence of the FCR-1B-d8 marker). This parameter change ensures consistent, reproducible results across different laboratories and conditions while maintaining methodological simplicity through standardized molecular protocols.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The dCAPS marker enables quick and stable prediction of wheat resistance to FCR, reducing identification time from months to days, conserving resources, and improving breeding efficiency by guiding hybrid combination preparation.
Implementation Method 1
utilizing a specific primer set for PCR amplification
Implementation Method 2
electrophoresis to identify resistance to Fusarium pseudograminearum
Data Source
AI summary
A derived cleaved amplified polymorphic sequence (dCAPS) molecular marker for identifying resistance of wheat to Fusarium crown rot caused by Fusarium pseudograminearum, primers and an application thereof are provided, relating to the field of wheat disease-resistant breeding and biotechnologies. The dCAPS molecular marker is located at a site of 676077050 to 676084414 base pairs (bp) on wheat chromosome 1B. A primer set for identifying the dCAPS molecular marker is provided. The resistance to Fusarium crown rot caused by Fusarium pseudograminearum can be rapidly predicted and screened through the molecular marker detection during seedling stage, which saves precious scientific research time and a lot of manpower and material resources, and the identification result is stable. Therefore, the method can accurately and efficiently screen a wheat variety with resistance to the Fusarium crown rot, and greatly improve a breeding process of a wheat with high-yield and resistance to the Fusarium crown rot.