Antimicrobial Susceptibility Test Using Dead Bacteria ATP Luminescence
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Solution Overview
Problem
Current antimicrobial susceptibility tests using the ATP method require multiple measurements to determine live bacteria growth, making the process time-consuming and labor-intensive, and it is difficult to quickly obtain results.
Innovation Solution
An antimicrobial susceptibility test device that measures ATP luminescence in a culture liquid containing bacteria, using a determination unit to assess antimicrobial susceptibility based on the ATP luminescence amount derived from dead bacteria, allowing for quicker and easier testing.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If multiple measurements of live bacteria ATP are performed to determine bacteria growth, then measurement precision is improved, but loss of time and productivity deteriorate
Solution Approach 1:
The invention extracts and measures only the critical indicator (dead bacteria ATP) rather than performing multiple measurements of live bacteria ATP. By focusing on the ATP remaining after live bacteria are eliminated, the method obtains sufficient measurement precision with a single measurement, thereby resolving the contradiction between measurement accuracy and time consumption.
2Reliability
If multiple measurements are conducted to evaluate bacteria growth, then reliability of result is improved, but device complexity and operation difficulty increase
Solution Approach 1:
The method extracts the essential information needed for reliable test results by measuring only dead bacteria ATP after eliminating live bacteria. This extraction approach maintains result reliability while simplifying the measurement process and reducing operational complexity compared to multiple sequential measurements.
3Measurement precision
If live bacteria ATP is measured multiple times during culture, then bacteria growth evaluation accuracy is improved, but ease of operation deteriorates
Solution Approach 1:
The invention simplifies operation by extracting and measuring a single indicator (dead bacteria ATP) rather than requiring multiple sample preparations and measurements. The method eliminates live bacteria first, then measures the remaining ATP once, making the process easier to operate while maintaining evaluation accuracy.
4Measurement precision
If ATP measurement is performed multiple times to determine bacteria growth, then measurement precision is improved, but productivity deteriorates
Solution Approach 1:
The method extracts the critical measurement target (dead bacteria ATP) and determines bacteria growth with a single measurement. This extraction approach maintains measurement precision while significantly improving test throughput and productivity by eliminating the need for multiple sequential measurements.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables prompt and accurate administration of antibiotics by quickly determining antimicrobial susceptibility through measuring dead bacteria ATP, reducing the need for frequent sample preparation and labor-intensive processes.
Implementation Method 1
The ATP method is used in detecting the ATP existing as the energy source inside the bacteria by using firefly-derived enzyme luciferase. The luciferase oxidizes luciferin which serves as a substrate in the presence of ATP and Mg2+ inside the bacteria, and a luminescence amount generated at that time is proportional to an ATP amount.
Data Source
AI summary
When bacteria growth is determined in the related art using an ATP method, the bacteria growth is determined, based on an increase or a decrease in live bacteria ATP with the lapse of a culture time. Accordingly, it is necessary to measure the live bacteria ATP multiple times while antimicrobial susceptibility culture is carried out. It takes time and labor in sample preparation for each measurement, and it is difficult to quickly obtain an antimicrobial susceptibility result. Therefore, according to the present invention, the presence or absence of antimicrobial susceptibility of bacteria is determined, based on an ATP luminescence amount derived from dead bacteria in a culture liquid.