Decellularized Adipose Tissue Scaffold for Soft Tissue Reconstruction
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Solution Overview
Problem
Current methods for soft tissue reconstruction, such as autologous fat transfer, face challenges including variable persistence, donor site morbidity, and post-operative calcifications, which can interfere with mammography readings and require multiple procedures, and are limited by the patient's adipose volume, especially in cancer patients undergoing chemotherapy or radiation.
Innovation Solution
Processed adipose tissue compositions are developed, including decellularized adipose tissue extracellular matrix that viable cells can attach to, which may include cross-linking agents, biopolymers, and other additives, and are prepared through methods like homogenization and nuclease treatment to reduce immunogenicity and lipid content, creating a biocompatible scaffold for tissue reconstruction.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If autologous fat transfer is performed, then tissue restoration is achieved, but persistence varies widely (30-90%) and multiple procedures are required
Solution Approach 1:
The patent extracts and removes cellular components from adipose tissue through decellularization processes, isolating the extracellular matrix and specific cell populations (stromal vascular fraction) that provide structural support and regenerative capacity while removing elements that cause variability and degradation
Solution Approach 2:
The patent applies chemical treatments (enzymes, detergents, cross-linking agents) and physical processes (supercritical fluid extraction, freeze-drying) to transform adipose tissue into a stable, standardized product with controlled properties that ensure consistent persistence and performance
2Reliability
If muscle tissue is transferred for breast reconstruction, then reconstruction is achieved, but donor site morbidity and extended recovery time occur
Solution Approach 1:
The patent extracts the beneficial components of adipose tissue (extracellular matrix, stromal vascular fraction cells) and separates them from the need for large-volume donor site harvesting, allowing reconstruction with minimal donor site impact
Solution Approach 2:
The patent creates a composite material combining decellularized adipose extracellular matrix with viable stromal vascular fraction cells, producing a tissue-engineered construct that provides structural support and regenerative capacity without the morbidity of traditional muscle flap transfers
3Reliability
If adipose tissue is implanted, then tissue volume is restored, but post-operative calcifications occur that interfere with mammography
Solution Approach 1:
The patent removes calcification-prone cellular components and lipid content through decellularization and extraction processes, leaving a purified extracellular matrix that does not undergo pathological calcification while maintaining tissue restoration capabilities
Solution Approach 2:
The patent chemically modifies the adipose tissue through cross-linking and purification processes that alter its composition to prevent calcification while preserving the structural and aesthetic properties needed for tissue restoration
4Adaptability or versatility
If patient's adipose volume is limited, then autologous fat transfer is constrained, but processed adipose tissue can be expanded
Solution Approach 1:
The patent performs preliminary harvesting and processing of adipose tissue from available donor sites, concentrating the beneficial components into a standardized product that can be stored and used when needed, allowing treatment of patients who would otherwise be ineligible due to insufficient adipose volume
Solution Approach 2:
The patent creates a concentrated composite material from processed adipose tissue that provides enhanced volume and consistency, allowing reconstruction in patients with limited native adipose reserves by combining decellularized matrix with viable cell populations
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The processed adipose tissue compositions provide a predictable, long-lasting, and biocompatible material for soft tissue reconstruction, reducing the need for multiple procedures and minimizing donor site morbidity, while maintaining the native architecture and bioactivity of adipose tissue, suitable for clinical use in reconstructive and cosmetic surgeries.
Implementation Method 1
manipulating the adipose with one or more buffers sequentially to promote lipid and cell removal to prepare processed adipose tissue
Implementation Method 2
the decellularized adipose includes cross-linking agent
Data Source
AI summary
The invention provides compositions and methods for the preparation of processed adipose tissue. The invention further provides methods of use of the processed adipose tissue.