Decellularized ECM Tissue Paper Freeze-Drying
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Solution Overview
Problem
Existing methods for preparing decellularized extracellular matrix (dECM) materials often damage extracellular matrix components and reduce bioactivity due to the use of acids, enzymes, or organic solvents, resulting in materials with limited controllability and mechanical strength, which hampers their effectiveness in tissue regeneration.
Innovation Solution
A novel preparation method for dECM tissue paper that involves decellularizing fresh tissue using a mild process without acids, enzymes, or organic solvents, followed by homogenization, air-drying, freeze-drying, and cross-linking with ethanol solutions, allowing for the retention of native ECM components and activity, and enabling the creation of tissue paper with controllable thickness and mechanical strength.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If acids, enzymes, or organic solvents are used to process dECM materials, then the extracellular matrix components can be dissolved or digested, but the bioactivity of the ECM components is damaged or lost
Solution Approach 1:
The patent replaces chemical processing methods (acids, enzymes, organic solvents) with a physical filtration system. The decellularized extracellular matrix is filtered through a filtration device with controllable pore sizes to separate and purify ECM components without chemical degradation, thereby maintaining bioactivity while achieving effective processing
Solution Approach 2:
The patent introduces a filtration device as an intermediary medium between the decellularized tissue and the final ECM product. This physical intermediary allows selective separation and purification of ECM components through pore filtration, avoiding direct chemical contact that would damage bioactivity
2Ease of manufacture
If dECM materials are directly cross-linked after decellularization, then the material structure is formed, but the macro and micro structures lack controllability
Solution Approach 1:
The patent controls the pore size parameter of the filtration device to precisely regulate the macro and micro structure of the dECM material. By adjusting the filtration pore dimensions, the resulting tissue paper achieves controllable porosity, thickness, and structural architecture while maintaining material integrity
Solution Approach 2:
The patent segments the decellularized extracellular matrix into controlled structural components through filtration. The filtration process separates ECM components based on size and morphology, creating a segmented structure with controllable pore distributions at multiple scales
3Strength
If dECM products are made compact to improve structural integrity, then mechanical strength is enhanced, but cell migration and tissue regeneration are limited
Solution Approach 1:
The patent utilizes porous filtration to create a highly porous tissue paper structure with controlled pore sizes. This porous architecture provides both structural integrity through the ECM matrix and facilitates cell migration through interconnected pores, resolving the contradiction between compactness and biological activity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method effectively retains ECM components and activity, enhancing the mechanical properties and controllability of the dECM tissue paper, expanding its application in tissue repair and regeneration while simplifying industrial production.
Implementation Method 1
freeze-drying the homogenates to obtain the tissue paper
Implementation Method 2
freeze-drying the homogenates
Implementation Method 3
cross-linking the tissue paper obtained in step (2) in a cross-linking solution
Data Source
AI summary
The present disclosure discloses a preparation method and application of decellularized extracellular matrix tissue paper, and belongs to the technical field of regenerative medicine. The preparation method includes the following steps: (1) preparing decellularized extracellular matrix materials; (2) preparing tissue paper: homogenizing and stirring the prepared decellularized extracellular matrix materials, then, placing the obtained homogenates on a flat-bottom filter screen to filter out water, standing and air-drying the filtered homogenates, and freeze-drying the homogenates to obtain the tissue paper; (3) cross-linking the tissue paper: cross-linking the tissue paper in a cross-linking solution; and (4) freeze-drying or stacking and compacting the cross-linked tissue paper. According to the present disclosure, the decellularized extracellular matrix tissue paper with tissue specificity is prepared by a traditional paper-making technology combined with a freeze-drying technology.