Decellularized Extracellular Matrix Spheroid Scaffold

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Solution Overview

Problem

Current biomedical scaffolds, whether artificial or derived from natural polymers or decellularized biological tissues, fail to effectively replicate the microstructure and bioactivity of natural extracellular matrices, limiting their ability to support cell growth and tissue regeneration, and face challenges such as immune rejection, batch variability, and size limitations.

Innovation Solution

A decellularized extracellular matrix is produced using a three-dimensional cell spheroid culture method, involving alkaline non-ionic surfactants and deoxyribonuclease treatment, which includes bioactive components like growth factors and ECM proteins, and can be customized in size and composition to promote tissue regeneration.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If decellularized tissues are used as bioactive scaffolds, then biological activity is improved, but immune rejection and disease transmission risks occur

Engineering Contradiction:

ImprovebioactivityVSAvoidimmune rejection

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent extracts only the essential bioactive components (ECM proteins, growth factors) from cellular structures through decellularization, removing DNA and cellular antigens that cause immune rejection while preserving the beneficial biological activity needed for tissue regeneration

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The patent creates a simplified copy of natural ECM by decellularizing cultured tissue, replicating the essential bioactive structure and composition without the harmful cellular components, thereby maintaining functionality while eliminating immune risks

Inventive Principle:

Principle #26Copying

2Loss of time

If two-dimensional cell culture is used to produce dECM, then production time is reduced, but the resulting matrix is thin, fragile, and lacks three-dimensional microstructure

Engineering Contradiction:

Improveproduction timeVSAvoidthree-dimensional structure

Core Design Contradiction:

Loss of timeVSShape

Solution Approach 1:

The patent transitions from two-dimensional cell culture to three-dimensional spheroid culture, enabling the production of dECM with authentic three-dimensional microstructure, porosity, and mechanical properties while maintaining efficient production timelines

Inventive Principle:

Principle #17Another dimension (Dimensionality change)

3Reliability

If natural polymers are used to construct ECM, then biocompatibility is improved, but the complex structure cannot be perfectly simulated

Engineering Contradiction:

ImprovebiocompatibilityVSAvoidmicrostructure simulation

Core Design Contradiction:

ReliabilityVSManufacturing precision

Solution Approach 1:

The patent allows cells to self-organize and naturally deposit ECM components during three-dimensional culture, creating an authentic, cell-generated matrix that perfectly replicates natural tissue microstructure without requiring complex artificial construction

Inventive Principle:

Principle #25Self-service

4Reliability

If decellularized scaffolds are derived from biological tissues, then bioactivity is improved, but batch variability and quality differences occur

Engineering Contradiction:

ImprovebioactivityVSAvoidbatch consistency

Core Design Contradiction:

ReliabilityVSStability of the object's composition

Solution Approach 1:

The patent standardizes key parameters including cell type, culture conditions, spheroid size, and decellularization protocols to ensure consistent bioactive composition across batches, while maintaining the natural ECM structure that provides biological activity

Inventive Principle:

Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The resulting decellularized extracellular matrix closely mimics natural tissue structures, supports cell proliferation, and enhances angiogenesis, allowing for scalable and customizable scaffold production that can be used for tissue repair and regeneration with reduced immune rejection risks.

Implementation Method 1

subjecting a decellularization treatment to the three-dimensional cell spheroid by using an alkaline non-ionic surfactant and a deoxyribonuclease

Methodology Applied

Scientific EffectDecellularization treatment:

Data Source

PatentUS12097303B2Decellularized extracellular matrix, preparation process and uses thereof

Publication Date: 2024.09.24 NATIONAL TSING HUA UNIVERSITY

AI summary

The present disclosure provides a decellularized extracellular matrix, the preparation process and uses thereof. The decellularized extracellular matrix of the present disclosure is derived from a three-dimensional cell spheroid, and the decellularized extracellular matrix has a three-dimensional spherical structure. The decellularized extracellular matrix of the present disclosure can be used to prepare a biomedical material scaffold for promoting tissue regeneration and repair.