Decellularized Muscle Matrix Implants for Regeneration
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Solution Overview
Problem
Current muscle regenerative procedures face challenges such as excess inflammation, scar tissue formation, and inadequate muscle regeneration due to the use of muscle transplants and completely decellularized matrices, which lack strength and effectiveness in repairing muscle tissue.
Innovation Solution
Development of muscle implants comprising decellularized muscle matrices that retain 20-80% of myofibers, combined with decellularized dermal matrices, using trypsin and decellularization solutions like TRITON X-100, and processed to form particulate or bilayer implants for improved muscle repair and regeneration.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If muscle transplants are used for muscle repair, then muscle regeneration is achieved, but excess inflammation and scar tissue formation occur
Solution Approach 1:
The patent extracts and removes cellular components (nuclei, mitochondria, cytoplasm) from donor muscle tissue through decellularization processes, retaining only the extracellular matrix scaffold. This eliminates immunogenic cellular elements that cause inflammation and rejection while preserving the structural framework needed for muscle regeneration.
Solution Approach 2:
The decellularized muscle matrix serves as an intermediary scaffold between the host tissue and the muscle defect. It provides a biocompatible framework that guides host cell infiltration and muscle regeneration without triggering the harmful immune responses associated with intact allografts.
2Object-generated harmful factors
If completely decellularized matrices are used for muscle repair, then inflammation is reduced, but strength and effectiveness are lost
Solution Approach 1:
The patent optimizes decellularization parameters (detergent concentration, exposure time, temperature) to achieve a balance where cellular components are sufficiently removed to minimize inflammation while preserving critical structural proteins and matrix integrity that provide mechanical strength.
Solution Approach 2:
The resulting decellularized muscle matrix is a composite structure containing preserved extracellular matrix components (collagen, elastin, glycoproteins) combined with retained myofiber structures. This composite maintains both biocompatibility and mechanical properties necessary for effective muscle repair.
3Reliability
If muscle allografts are harvested from donor sites, then muscle repair is achieved, but muscle loss occurs at the donor site
Solution Approach 1:
The patent employs a disposable decellularized muscle matrix scaffold that is implanted and then gradually degraded and absorbed by the host tissue. This eliminates the need for harvesting functional muscle tissue from donors, as the scaffold serves its purpose and is subsequently replaced by regenerating host muscle.
Solution Approach 2:
The decellularized matrix acts as a temporary intermediary that facilitates muscle regeneration at the defect site without requiring permanent preservation of donor muscle tissue. The scaffold is eventually resorbed and replaced by host-generated muscle, eliminating the trade-off between donor site muscle loss and recipient muscle gain.
4Strength
If intact muscle tissue is used for repair, then structural strength is maintained, but excessive inflammation and rejection occur
Solution Approach 1:
The patent extracts and removes immunogenic cellular components (nuclei, mitochondria, cytoplasm) from intact muscle tissue through decellularization, retaining only the acellular extracellular matrix scaffold. This eliminates the immune recognition triggers that cause inflammation and rejection while preserving the structural integrity provided by the matrix.
Solution Approach 2:
The patent segments the muscle tissue into two functional components: cellular elements (removed to eliminate immune responses) and extracellular matrix (retained to provide structure). This segmentation allows each component to fulfill its appropriate function without the harmful interactions that occur in intact tissue.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The muscle implants promote increased native muscle regeneration, reduce scar tissue formation, and enhance muscle volume and strength, offering a more effective long-term management of muscle defects and injuries compared to existing methods.
Implementation Method 1
contacting the at least one muscle sample with a trypsin solution; decellularizing the at least one muscle sample
Implementation Method 2
the decellularization solution comprises at least one of TRITON X-100, sodium dodecyl sulfate, sodium deoxycholate, and polyoxyethylene (20) sorbitan monolaurate
Data Source
AI summary
Disclosed herein are muscle implants and methods of making muscle implants comprising one or more decellularized muscle matrices. The muscle matrices can, optionally, be joined to one or more decellularized dermal matrices. The muscle implants can be used to enhance muscle volume or to treat muscle damage, defects, and/or disorders. The decellularized muscle matrices in the implants retain at least some of the myofibers found in a muscle tissue prior to processing.