Decellularized Omentum Spherical Particles for Cell Viability

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Solution Overview

Problem

Existing cell encapsulation technologies face challenges in supporting cellular viability and function over extended periods, leading to inconsistent results in preclinical and clinical trials.

Innovation Solution

The development of spherical particles comprising decellularized omentum, which are used for cell and/or biomolecule delivery. These particles are generated by dispersing solubilized decellularized omentum in oil and heating it to form solid particles, which can encapsulate cells and biomolecules.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If existing cell encapsulation technologies are used, then cell delivery is achieved, but cellular viability and function deteriorate over extended periods

Engineering Contradiction:

Improvecellular viabilityVSAvoidextended period

Core Design Contradiction:

ReliabilityVSDuration of action of moving object

Solution Approach 1:

The patent uses decellularized omentum, a natural extracellular matrix material, as the encapsulation medium. This composite material provides a biocompatible environment that maintains cellular viability and function over extended periods, resolving the contradiction between reliability and duration by offering both short-term protection and long-term sustenance.

Inventive Principle:

Principle #40Composite materials

Solution Approach 2:

The patent modifies the physical and chemical parameters of the encapsulation environment by using decellularized omentum with specific properties (porosity, mechanical strength, biochemical composition). These parameter changes create optimal conditions for cell survival and function maintenance over time, addressing the contradiction between sustained viability and extended duration.

Inventive Principle:

Principle #35Parameter changes

2Reliability

If existing cell encapsulation technologies are used, then cell delivery is achieved, but functional consistency deteriorates leading to inconsistent results

Engineering Contradiction:

Improvefunctional consistencyVSAvoidinconsistent results

Core Design Contradiction:

ReliabilityVSManufacturing precision

Solution Approach 1:

The patent employs decellularized omentum as a homogeneous natural matrix that provides uniform biochemical and mechanical properties throughout the encapsulation structure. This homogeneity ensures consistent cell behavior and function across different encapsulated cells, resolving the contradiction between functional consistency and manufacturing precision by eliminating variability in the encapsulation environment.

Inventive Principle:

Principle #33Homogeneity

Solution Approach 2:

The decellularized omentum matrix provides self-organizing properties and inherent biochemical signals that guide cell behavior without requiring precise external control. This self-service capability ensures consistent functional outcomes by allowing cells to naturally interact with the matrix properties, reducing variability in results.

Inventive Principle:

Principle #25Self-service

3Reliability

If decellularized omentum particles are used for cell encapsulation, then cellular viability is improved, but particle size control becomes critical

Engineering Contradiction:

Improvecellular viabilityVSAvoidparticle size

Core Design Contradiction:

ReliabilityVSManufacturing precision

Solution Approach 1:

The patent controls particle size by adjusting parameters during the emulsification and solidification process, including oil phase composition, surfactant concentration, and temperature. These parameter changes enable precise control over particle diameter while maintaining the beneficial properties of decellularized omentum for cell viability.

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The patent replaces mechanical cell encapsulation methods with a chemical/emulsion-based approach where decellularized omentum forms particles through controlled emulsification and solidification. This substitution allows for more precise particle size control through chemical parameters rather than mechanical forces, resolving the contradiction between viability improvement and size precision.

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The particles provide enhanced cellular viability and controlled release of encapsulated cargo, improving the efficiency of cell transplantation and tissue engineering applications.

Implementation Method 1

heating the emulsified, decellularized omentum to generate solid particles of decellularized omentum

Methodology Applied

Scientific EffectHeating: Heating

Data Source

PatentUS12311075B2Particles comprising decellularized omentum

Publication Date: 2025.05.27 RAMOT AT TEL AVIV UNIVERSITY LTD

AI summary

A spherical particle comprising decellularized omentum being between 1 nM-300 μM in diameter is disclosed. In some embodiments, the particle comprises biological cells. In other embodiments, the particle comprises a biomolecule. Uses of the particles are also disclosed.