Decellularized Small Particle Tissue via Sequential Filtration
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Solution Overview
Problem
Current methods for producing decellularized tissues face challenges in removing cells while preserving beneficial proteins, often leaving behind undesirable debris, and struggle to create small particle tissues suitable for various medical applications.
Innovation Solution
A novel process involving blood-laden tissues, using sequential particle size separation and recycling systems with decellularizing agents like hydrogen peroxide, allows for efficient removal of cellular components and debris, retaining beneficial proteins, and producing small particle decellularized tissue that can be injectible or molded into various shapes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Object-generated harmful factors
If decellularizing agents such as hydrogen peroxide, detergents, or enzymes are used to remove cells from tissue, then cellular materials are removed, but beneficial proteins are also lost
Solution Approach 1:
The decellularization process is divided into multiple sequential steps with different agents and conditions. First, mild detergents remove cells while preserving proteins, then controlled oxidation with hydrogen peroxide removes remaining cellular materials, and finally enzymes selectively digest residual debris. This segmentation allows selective removal of harmful cellular materials while preserving beneficial proteins at each stage.
Solution Approach 2:
The patent employs systematic changes in chemical parameters including pH adjustments, controlled oxidation levels, and sequential concentration variations of decellularizing agents. By carefully controlling these parameters, the process achieves selective removal of cellular materials while maintaining beneficial proteins intact throughout the decellularization sequence.
2Object-generated harmful factors
If harsh decellularizing materials such as sodium hydroxide or hydrochloric acid are used, then cellular remnants are removed, but the process requires extreme pH conditions and yields unsuitable results
Solution Approach 1:
The patent uses a sequence of relatively mild, consumable decellularizing agents including detergents, hydrogen peroxide, and enzymes that can be easily removed or neutralized. These agents act as disposable tools that perform their decellularization function and are then eliminated, leaving no harsh residues that would compromise tissue suitability for medical applications.
Solution Approach 2:
The patent converts the potentially harmful effects of oxidation by using controlled, sequential exposure to hydrogen peroxide at controlled concentrations. The oxidation that would normally damage proteins is instead harnessed to selectively remove cellular materials, with the process conditions optimized so that beneficial proteins remain intact while cellular debris is oxidized and removed.
3Object-generated harmful factors
If traditional decellularization methods are used, then cells are removed, but the tissue cannot be produced as small particles suitable for injection or molding
Solution Approach 1:
The decellularized tissue is processed through sequential size reduction steps including homogenization, grinding, and filtration to produce controlled particle size distributions. This segmentation of the tissue into small particles maintains the decellularized state while creating versatile forms that can be injected, molded, or applied as coatings for various medical applications.
Solution Approach 2:
The patent controls physical parameters including particle size distribution, moisture content, and density through controlled drying and processing conditions. These parameter changes transform the decellularized tissue into small particles with specific physical properties suitable for injection, molding, or other customized delivery methods.
4Object-generated harmful factors
If batch type processes with much manipulation are used, then decellularization can be performed, but the process is unwieldy and inefficient
Solution Approach 1:
The patent employs continuous processing steps where decellularizing agents flow through the tissue material in a continuous manner rather than batch processing. This continuity eliminates repeated loading and unloading operations, maintains consistent chemical conditions throughout the process, and significantly improves throughput and efficiency while achieving complete decellularization.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The process effectively retains beneficial proteins and removes undesirable materials, resulting in a high-quality, versatile small particle decellularized tissue suitable for diverse medical uses, including wound healing, cell culture, and tissue regeneration, with reduced reagent consumption and process inefficiencies.
Implementation Method 1
using decellularizing agents like hydrogen peroxide
Data Source
AI summary
A process for producing a decellularized small particle tissue which involves selecting an appropriate tissue starting material from which a decellularized small particle tissue is desired to be prepared, treating the tissue with a decellularizing agent at an acid pH to remove at least a portion of the cellular material therefrom and to yield a product comprising a liquid component and a solid component, subjecting the liquid component and the solid component to a plurality of filters, F1 through Fn, wherein n may be 2, or an integer higher than 2, wherein the pore sizes of the filter F1 through Fn range from 200 microns to 10 Kilo Daltons, yielding filtrates and resonates, recycling either of said filtrates or said retentates or both, either separately or together, to any of steps b) or c) or both, at least one time, and isolating a decellularized small particle tissue from any of steps of the process. Both the product and process are novel.