Decellularized Tissue Impregnation via Pressure Cycling
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Solution Overview
Problem
Existing methods for filling biological tissues with functional substances often result in uneven distribution, with the substance primarily fixing in the surface layer, and can damage the tissue structure, making it difficult to use the tissue as a biological material for grafts.
Innovation Solution
A method involving a reduced pressure step followed by a pressurized step to fill animal-derived decellularized tissue materials with a liquid, ensuring even impregnation without altering the tissue structure, using conditions such as vacuum and high hydrostatic pressure to facilitate the permeation of the liquid into the tissue voids.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If a biological tissue is immersed in a liquid containing a functional substance, then the functional substance is introduced into the tissue, but the functional substance is fixed only in a surface layer and it is difficult to fill throughout the inside of the biological tissue
Solution Approach 1:
The patent applies periodic action by alternating between reduced pressure steps and pressurized steps multiple times. Each cycle draws liquid into the tissue voids during reduced pressure, then allows excess liquid to be removed during pressurization. Repeating this cycle ensures progressive and uniform penetration of functional substances throughout the entire tissue volume, overcoming the limitation of surface-only fixation in simple immersion methods.
Solution Approach 2:
The patent utilizes parameter changes by dynamically adjusting pressure conditions between reduced pressure (to draw liquid in) and pressurized states (to remove excess liquid and prevent over-saturation). This periodic parameter modulation enables controlled, uniform distribution of functional substances throughout the tissue interior while maintaining structural integrity, resolving the contradiction between achieving deep penetration and uniform distribution.
2Quantity of substance
If heat, microwave, ultrasound or the like is used to fill the biological tissue with liquid, then the liquid can be filled into the tissue, but the support tissue may be denatured and destroyed
Solution Approach 1:
The patent replaces thermal and acoustic energy fields (heat, microwave, ultrasound) with a mechanical pressure field system. By using controlled reduced pressure and pressurization cycles, the method achieves liquid penetration through mechanical pressure differentials rather than thermal or acoustic energy, thereby filling the tissue with liquid while preserving the structural integrity and preventing denaturation of the support tissue.
3Ease of operation
If a method of immersion is used to fill biological tissue with functional substance, then the process is simple to operate, but the functional substance is fixed only in a surface layer
Solution Approach 1:
The patent maintains ease of operation by using a simple immersion setup but enhances penetration depth through periodic action. The tissue remains immersed in the functional substance solution throughout the process, while periodic cycles of reduced pressure and pressurization drive the liquid progressively deeper into the tissue voids. This approach preserves operational simplicity while achieving thorough throughout-penetration that simple continuous immersion cannot achieve.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method allows for effective and even impregnation of decellularized tissue products with liquids, maintaining the tissue structure and enabling their use as functional grafts for transplantation.
Implementation Method 1
a reduced pressure step of bringing an animal-derived decellularized tissue material and a liquid into contact under a reduced pressure condition
Implementation Method 2
a pressurized step of bringing the same into contact under a pressurized condition subsequent to the reduced pressure step
Data Source
Figure 1
Figure 2A~2B
Figure 3A~3B
AI summary
Provided are: a method for preparing a decellularized tissue product in which decellularized tissue can be filled with liquid while changes in the structure of support tissue constituting the decellularized tissue are inhibited; and a graft provided with a decellularized tissue product. The method for preparing a decellularized tissue product includes a reduced-pressure step for bringing an animal-derived decellularized tissue material and a liquid into contact under reduced-pressure conditions, and/or a pressurized step for bringing same into contact under pressurized conditions. The graft is provided with a decellularized tissue product prepared by the method of preparation.