Degassed Cellular Disruption Media for Organ Decellularization
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Solution Overview
Problem
Current methods for decellularizing isolated organs are inefficient, requiring longer times and larger volumes of non-degassed cellular disruption media, which can lead to increased microbubble formation and reduced cellular removal efficiency.
Innovation Solution
The use of a degassed cellular disruption media, which is partially degassed to remove dissolved gases, allowing for reduced volumes and times to achieve decellularization, and the inclusion of radical-generating compounds like peracetic acid to enhance cellular removal without compromising the integrity of the extracellular matrix.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If non-degassed cellular disruption media is used for decellularization, then the decellularization process can be performed with standard procedures, but the process requires longer time and larger volumes of media, and leads to increased microbubble formation
Solution Approach 1:
The patent applies parameter changes by modifying the physical state of the cellular disruption media through degassing. By removing dissolved gases from the media before use, the patent changes a fundamental parameter of the liquid medium, which prevents microbubble formation during perfusion and allows for reduced processing time and volume while maintaining decellularization effectiveness.
Solution Approach 2:
The patent implements preliminary action by performing degassing of the cellular disruption media before the decellularization process begins. This pre-treatment step removes dissolved gases in advance, preventing subsequent microbubble formation during perfusion and enabling more efficient decellularization with shorter times and reduced media volumes.
2Ease of manufacture
If non-degassed cellular disruption media is used for decellularization, then standard procedures can be followed, but microbubble formation increases and cellular removal efficiency decreases
Solution Approach 1:
The patent modifies the physical parameter of the cellular disruption media by removing dissolved gases through degassing. This parameter change prevents microbubble formation during perfusion, thereby improving cellular removal efficiency without significantly complicating the overall procedure, as degassing is a straightforward pre-treatment step.
3Quantity of substance
If larger volumes of cellular disruption media are used, then decellularization can be performed with standard methods, but the process becomes less efficient and requires more time
Solution Approach 1:
The patent changes the physical parameter of the cellular disruption media through degassing, which enables more efficient cellular removal. This parameter modification allows the process to achieve effective decellularization with smaller media volumes and shorter times, thereby improving productivity without sacrificing cellular removal effectiveness.
4Reliability
If degassed cellular disruption media is used, then microbubble formation is reduced and cellular removal efficiency increases, but additional degassing equipment or steps are required
Solution Approach 1:
The patent performs degassing as a preliminary action before the decellularization process. This pre-treatment step, while adding a process step, uses simple and accessible degassing methods that do not significantly increase device complexity. The benefit of improved cellular removal efficiency and reduced microbubble formation outweighs the minor addition of a pre-treatment step.
5Duration of action of moving object
If longer contact time is used with non-degassed media, then decellularization can be achieved, but the process is less efficient and produces more microbubbles
Solution Approach 1:
The patent changes the physical parameter of the cellular disruption media by removing dissolved gases, which fundamentally alters the perfusion dynamics. This parameter change enables shorter contact times to achieve effective decellularization, thereby improving productivity without sacrificing cellular removal efficiency, as the degassed media prevents microbubble formation that would otherwise impede the process.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach results in faster and more efficient decellularization with reduced microbubble formation and higher cellular removal efficiency, while maintaining the structural integrity of the extracellular matrix, enabling quicker production of decellularized organs.
Implementation Method 1
a liquid can be at least partially degassed
Implementation Method 2
a liquid can comprise a detergent and/or a radical-generating compound
Implementation Method 3
a liquid can comprise a detergent and/or a radical-generating compound
Data Source
AI summary
Disclosed herein are compositions and methods to decellularize an isolated organ or portion thereof. Also disclosed herein are compositions and methods for treatment of disease utilizing a decellularized or recellularized organ. Also disclosed herein are methods of improving decellularization and/or recellularization of an isolated organ or portion thereof.