Degradable Carrier Cell Isolation via Fluoropolymer Bag
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Solution Overview
Problem
Existing cell culture and isolation methods face challenges in efficiently retaining desired cells without altering their function, particularly in the process of capturing and releasing cells from solid supports.
Innovation Solution
The use of containers with an inner surface comprising a fluoropolymer and containing a degradable carrier with biological agent-capturing moieties allows for the capture and separation of desired cells, which can be released without functional alteration by degrading the carrier.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If cells are anchored to a solid support for selective capture, then desired cells can be retained and separated from undesired cells, but the isolated cells may have altered function due to cleaving the anchoring protein
Solution Approach 1:
The patent introduces a degradable carrier as an intermediary between the solid support and the cells. The carrier presents biological agent-capturing moieties that bind to cells, allowing selective capture without direct anchoring to the support. This mediator approach enables cell retention while avoiding the functional alterations caused by direct solid support anchoring and subsequent cleaving.
Solution Approach 2:
The patent utilizes degradable properties of the carrier material, changing its physical or chemical state from intact to degraded form. This parameter change allows the carrier to maintain cells during capture, then be degraded to release cells without functional alteration, solving the contradiction between retention and functional integrity.
2Productivity
If conventional solid support anchoring is used for cell capture, then cell separation is achieved, but the process requires complex cleaving steps to release cells
Solution Approach 1:
The patent extracts the cell-capture function from the solid support system and transfers it to a degradable carrier. This extraction eliminates the need for complex cleaving steps required by conventional anchoring systems, as the carrier can be simply degraded rather than chemically cleaved, thereby reducing process complexity while maintaining isolation efficiency.
3Ease of operation
If cells remain suspended in the bag for easy removal with depleted medium, then operation is simple, but desired cells cannot be selectively retained
Solution Approach 1:
The patent segments the cell population into two groups: cells bound to the degradable carrier and cells remaining suspended in the medium. This segmentation enables selective retention of desired cells on the carrier while allowing easy removal of depleted medium with suspended cells, thus achieving both selective retention and operational simplicity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach improves the retention and isolation of desired cells while maintaining their functional integrity, and enhances the purity of isolated biological agents by minimizing adhesion of off-target agents.
Implementation Method 1
The inner surface of the container comprises a fluoropolymer
Implementation Method 2
the carrier comprises a plurality of biological agent-capturing moieties
Implementation Method 3
degrading the carrier
Data Source
AI summary
This disclosure relates to containers (e.g., in the form of bags) comprising a fluoropolymer and containing a degradable carrier comprising biological agent-capturing moieties, to cell isolation systems comprising such containers, and to methods for cultivating a biological agent using such containers. In one embodiment, the disclosure provides a container having an outer surface and an inner surface, the inner surface comprising a fluoropolymer, and contained in the container, a degradable carrier; wherein the carrier comprises a plurality of biological agent-capturing moieties.