Denitrifying Granular Sludge Formation for High-Nitrate Wastewater

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Solution Overview

Problem

Current methods struggle to rapidly form stable granular sludge for effective denitrification of high-nitrate nitrogen wastewater, as existing technologies face issues with microbial community stability, disintegration, and inefficiency under low C/N conditions, failing to meet stringent discharge standards.

Innovation Solution

A method utilizing the high adhesiveness of Actinobacillus succinogenes to promote denitrifying microorganisms, forming stable granular sludge by colonizing it into the sludge system, enhancing denitrification efficiency and reducing nitrite accumulation, without requiring pH control or additional carbon sources.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If aerobic granular sludge is used for denitrification, then denitrification capacity is improved, but granular structure stability deteriorates due to swelling and disintegration

Engineering Contradiction:
Improvedenitrification capacityVSAvoidgranular structure stability
Core Design Contradiction:
ProductivityVSStability of the object's composition

Solution Approach 1:

The patent introduces filamentous bacteria as intermediary organisms that act as a scaffold for granule formation. These filamentous bacteria create a three-dimensional network structure that traps and holds denitrifying bacteria, preventing their disintegration while maintaining high denitrification capacity. The filamentous bacteria serve as a structural mediator between the denitrifying bacteria and the external environment.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent creates a composite granular sludge system combining multiple functional components: filamentous bacteria providing structural framework, denitrifying bacteria providing metabolic function, and extracellular polymeric substances providing binding matrix. This composite structure integrates the advantages of each component while mitigating their individual weaknesses, achieving both stability and high denitrification capacity.

Inventive Principle:
Principle #40Composite materials

2Stability of the object's composition

If filamentous or polysaccharide-accumulating bacteria are used to form granular sludge, then granule formation is improved, but denitrification efficiency deteriorates due to competition for carbon sources

Engineering Contradiction:
Improvegranule formationVSAvoiddenitrification efficiency
Core Design Contradiction:
Stability of the object's compositionVSProductivity

Solution Approach 1:

The patent applies local quality differentiation within the granule structure. The filamentous bacteria are distributed throughout the granule framework, while denitrifying bacteria are concentrated in the internal matrix where carbon sources are abundant. This spatial differentiation allows filamentous bacteria to fulfill their structural role without excessively competing for carbon sources, while denitrifying bacteria access carbon sources locally for efficient denitrification.

Inventive Principle:
Principle #3Local quality

Solution Approach 2:

The patent segments the functional roles within the granular sludge: filamentous bacteria handle structural support and granule formation, while denitrifying bacteria handle nitrogen removal. This functional segmentation reduces direct competition for resources, as each group performs its specialized function in optimized conditions within the granule structure.

Inventive Principle:
Principle #1Segmentation

3Quantity of substance

If high concentration of nitrate nitrogen is treated, then treatment capability is improved, but microbial community stability deteriorates leading to system collapse

Engineering Contradiction:
Improvenitrate nitrogen removal capabilityVSAvoidmicrobial community stability
Core Design Contradiction:
Quantity of substanceVSReliability

Solution Approach 1:

The patent prepares the microbial community for high-nitrate treatment through gradual acclimation and by establishing a robust granular sludge structure with filamentous bacteria before full-load operation. The filamentous bacteria create a stable three-dimensional framework that can withstand high nitrate concentrations, cushioning the microbial community against the stress of high-nitrate shock and preventing system collapse.

Inventive Principle:
Principle #11Beforehand cushioning (Prior cushioning)

Solution Approach 2:

The patent utilizes parameter changes in the granular sludge structure, specifically the development of a three-dimensional filamentous network with increased surface area and porosity. This structural parameter change enables the system to accommodate high concentrations of nitrate nitrogen while maintaining microbial community stability, as the modified structure facilitates better mass transfer and reduces inhibitory effects of high nitrate levels.

Inventive Principle:
Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Rapidly forms stable denitrifying granular sludge that meets discharge standards, enhances denitrification efficiency, and reduces operational costs by shortening acclimation periods and eliminating the need for pH adjustments, while maintaining high denitrification activity and structural integrity.

Implementation Method 1

The succinic acid-producing microorganism Actinobacillus succinogenes and its promoting effect on denitrifying microorganisms

Methodology Applied
Scientific EffectMicrobial metabolism: Fermentation

Implementation Method 2

biological denitrification technology to facilitate the treatment of high-nitrate wastewater and achieve stable and efficient removal of nitrate nitrogen

Methodology Applied
Scientific EffectBiological denitrification: Reduction

Implementation Method 3

Granular sludge is a biological aggregate formed by the cohesion of various functional bacteria

Methodology Applied
Scientific EffectMicrobial cohesion: Adhesive

Data Source

PatentUS20260042690A1Method for rapid formation of denitrifying granular sludge and enhanced denitrification of high-nitrate nitrogen wastewater
Publication Date: 2026.02.12 TONGJI UNIV
  • US20260042690A1 patent drawing
  • US20260042690A1 patent drawing
  • US20260042690A1 patent drawing

AI summary

The present invention discloses a method for the rapid formation of denitrifying granular sludge and enhanced denitrification of high-nitrate nitrogen wastewater, belonging to the technical field of wastewater treatment. The method involves colonizing the succinic acid-producing microorganism A. succinogenes into sewage sludge. By leveraging its high adhesiveness and its promoting effect on denitrifying microorganisms, the method rapidly forms denitrifying granular sludge while simultaneously enhancing the denitrification of high-nitrate nitrogen wastewater. The method is simple to operate, requires no additional pH control, and generates no secondary pollution. It enables the rapid formation of stable, full-process denitrifying granular sludge, which reduces the system's acclimation period to high-nitrate wastewater, shortens the sludge cultivation cycle, and minimizes the accumulation and discharge of intermediate products, such as nitrite, during denitrification.