Deoxyribose-phosphate aldolase variant for L-glutamic acid production
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for producing L-glutamic acid through microbial fermentation are limited by the efficiency of enzymes and proteins involved in the biosynthetic pathway, requiring further research to enhance productivity.
Innovation Solution
A novel deoxyribose-phosphate aldolase variant with an altered amino acid sequence, specifically SEQ ID NO: 2, is introduced into a Corynebacterium sp. microorganism to enhance the production of L-glutamic acid by modifying the enzymatic activity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If wild-type strains or conventional mutant strains are used for L-glutamic acid production, then the production process is established, but the productivity is limited and requires further improvement
Solution Approach 1:
The patent applies parameter changes by mutating the deoxyribose-phosphate aldolase gene (deoA) to create a variant with altered amino acid sequence (SEQ ID NO: 2). This genetic parameter change results in an enzyme variant that improves carbon source flux through the pentose phosphate pathway, thereby increasing L-glutamic acid productivity by up to 10.4% compared to the parent strain
2Productivity
If genetic recombination technology is applied to modify enzymes and transcription factors, then L-glutamic acid productivity can be increased, but the complexity of research and development increases due to the large number of proteins involved
Solution Approach 1:
The patent applies the extraction principle by focusing on a single key enzyme - deoxyribose-phosphate aldolase - rather than attempting to modify multiple proteins simultaneously. By extracting and targeting this specific enzyme in the pentose phosphate pathway, the patent simplifies the research approach while achieving measurable productivity improvement, avoiding the complexity of screening and optimizing dozens of different proteins
3Productivity
If the enzymatic activity in the biosynthetic pathway is enhanced, then L-glutamic acid production efficiency is improved, but the understanding and optimization of metabolic pathways require extensive research
Solution Approach 1:
The patent applies preliminary action by proactively designing and implementing a specific deoxyribose-phosphate aldolase variant with a predetermined amino acid sequence (SEQ ID NO: 2) that is known to improve carbon source flux. This preliminary genetic modification eliminates the need for extensive time-consuming research and screening, as the variant's effectiveness in enhancing L-glutamic acid production is already established
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The introduction of the deoxyribose-phosphate aldolase variant increases L-glutamic acid productivity by up to 10.4% compared to the parent strain, indicating improved carbon source flux in the biosynthetic pathway.
Implementation Method 1
The deoxyribose-phosphate aldolase in the present invention is an enzyme that reversibly catalyzes the reaction of converting 2-deoxy-D-ribose 5-phosphate to D-glyceraldehyde 3-phosphate and acetaldehyde
Implementation Method 2
L-glutamic acid is a typical amino acid that is produced by microbial fermentation
Data Source
Figure 1
AI summary
The present invention relates to a Corynebacterium sp. mutant microorganism producing L-glutamic acid and a method of producing L-glutamic acid using the same, and more specifically, to a novel deoxyribose-phosphate aldolase variant involved in the L-glutamic acid biosynthetic pathway, a polynucleotide, and a transformant, as well as a method of producing L-glutamic acid using the same. The deoxyribose-phosphate aldolase variant according to the present invention is obtained by deleting one or more amino acids in the amino acid sequence constituting deoxyribose-phosphate aldolase to change the enzymatic activity of the deoxyribose-phosphate aldolase, and a recombinant microorganism comprising the deoxyribose-phosphate aldolase variant is capable of efficiently producing L-glutamic acid.