Depth Filter Layer with Inorganic Layer Double Hydroxide for DNA Depletion
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Solution Overview
Problem
Current depth filters are inadequate in efficiently removing undesired biological contaminants like DNA from cell culture solutions while allowing therapeutic proteins to pass through unimpeded, leading to increased costs and complexity in downstream purification processes.
Innovation Solution
Development of depth filter layers containing inorganic layer double hydroxides, specifically hydrotalcite, combined with cellulose fibers and positively charged wet strength agents, which selectively bind DNA without retaining therapeutic proteins, enhancing DNA depletion capacity up to 10 times compared to existing technologies.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If conventional depth filters (cellulose fibers with kieselguhr/perlite) are used, then cell and cell fragment separation is achieved, but DNA removal capacity is insufficient
Solution Approach 1:
The patent combines cellulose fibers, kieselguhr or perlite, and inorganic layer double hydroxide particles into a composite depth filter material. This composite structure integrates the mechanical filtration capability of cellulose with the high DNA adsorption capacity of LDH, achieving superior DNA removal while maintaining structural integrity and flow properties.
Solution Approach 2:
The patent modifies the chemical composition parameters of the depth filter by incorporating inorganic layer double hydroxide particles with specific surface areas and charge densities. This parameter change transforms the filter's adsorption characteristics, enabling high-capacity DNA binding through electrostatic interactions while preserving the physical filtration function.
2Quantity of substance
If more aggressive purification methods are used to remove DNA, then contaminant removal improves, but therapeutic protein loss increases and process costs rise
Solution Approach 1:
The inorganic layer double hydroxide particles provide localized positive charge sites within the filter matrix that specifically attract and bind negatively charged DNA molecules. This localized charge distribution enables selective DNA adsorption without creating uniform conditions that would cause non-specific binding of therapeutic proteins throughout the filter structure.
Solution Approach 2:
The patent utilizes the electrostatic properties of inorganic layer double hydroxide to convert the harmful effect of DNA contamination into a beneficial adsorption mechanism. The positively charged LDH particles naturally attract and capture negatively charged DNA, transforming the contamination problem into a self-selective purification process that spares therapeutic proteins.
3Quantity of substance
If filter media with higher adsorption capacity is used, then DNA depletion improves, but flow rate and protein passage are reduced
Solution Approach 1:
The filter media is segmented into distinct functional components: cellulose fibers provide the mechanical framework and bulk flow pathways, while dispersed inorganic layer double hydroxide particles provide localized DNA adsorption sites. This segmentation allows DNA binding to occur at specific locations without blocking overall flow through the filter matrix, maintaining high flow rates despite enhanced adsorption capacity.
Solution Approach 2:
The depth filter utilizes a porous structure where inorganic layer double hydroxide particles are distributed within the cellulose-kieselguhr matrix. The porous architecture provides extensive surface area for DNA adsorption while maintaining open channels for fluid flow, ensuring that high DNA depletion capacity does not compromise filtration throughput.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The new depth filter layers achieve significant DNA depletion while maintaining the integrity and passage of therapeutic proteins, simplifying downstream processes and reducing contaminant concentrations, thus reducing the need for additional costly purification steps and ensuring physicochemical stability for use before or after chromatography.
Implementation Method 1
at least one undesired biological component is adsorptively bound to the depth filter and is thus removed from the process solution
Data Source
AI summary
The present invention relates to a depth filter layer with inorganic layer double hydroxide, a method for production thereof, a filtration device containing this, a method for the separation of at least one contaminant from at least one target product in a liquid medium by means of at least one upstream depth filter layer and the use of the depth filter layer for removal of contaminants. The depth filter layer selectively retains contaminants such as nucleic acids, while target proteins of biotechnological processes are transmissible into the filtrate.