Depth Filter Extrusion for Cell-Derived Vesicle Production
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Solution Overview
Problem
Current methods for manufacturing cell-derived vesicles are limited by low production scale and complexity, with centrifuges being expensive and inefficient.
Innovation Solution
A method using a depth filter to extrude a cell culture solution, which includes harvesting cells, resuspending them, and extruding through the filter in a single device to produce cell-derived vesicles, reducing contamination risk and increasing production scale.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If a centrifuge is used to obtain extracellular vesicle mimetics, then the method is established, but the equipment is expensive and the yield is insufficient
Solution Approach 1:
The patent extracts and removes cell membranes from the cell culture solution by passing it through a depth filter with specific pore sizes (0.03-1 μm). This extraction process isolates the extracellular vesicles from the cellular debris and media, achieving high yield without requiring expensive centrifuge equipment. The depth filter selectively extracts vesicles based on their size and membrane structure.
Solution Approach 2:
The patent employs disposable depth filters as a low-cost alternative to expensive centrifuges. The depth filter is a simple, affordable device that can be easily discarded after use, eliminating the need for costly equipment maintenance and reducing overall production costs while maintaining high vesicle yield.
2Manufacturing precision
If multiple steps are used for harvesting, resuspending, and extruding cells, then the process can be completed, but the production process becomes complex and contamination risk increases
Solution Approach 1:
The patent merges the harvesting, resuspending, and extruding steps into a single integrated process. Cells are harvested by passing cell culture solution through a depth filter, then immediately resuspended and extruded through the same or a different depth filter in a continuous operation. This consolidation reduces the number of process steps, minimizes contamination risk, and maintains uniformity of the resulting cell-derived vesicles.
Solution Approach 2:
The depth filter serves multiple functions: it acts as a harvesting tool to separate cells from media, a resuspension device to redistribute cells uniformly, and an extrusion tool to force cells through the filter membrane for vesicle formation. This multi-functionality eliminates the need for separate equipment for each step, simplifying the overall process while maintaining manufacturing precision.
3Reliability
If cells are processed in multiple separate steps, then each step can be optimized, but the risk of external contamination increases
Solution Approach 1:
The patent implements continuous processing where cell culture solution is continuously passed through the depth filter for harvesting, followed by immediate continuous resuspension and extrusion. This continuous action eliminates idle time between steps, reduces exposure to the external environment, and maintains a closed system that minimizes contamination risk while ensuring high purity of the final cell-derived vesicles.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables stable, economic, and large-scale production of cell-derived vesicles with uniform characteristics, such as shape, size distribution, and protein and DNA concentrations, while minimizing external contamination.
Implementation Method 1
a depth filter through which a cell culture solution passes
Data Source
AI summary
The present invention relates to a method for manufacturing cell-derived vesicles using a depth filter. The present invention has the effect of enabling cell-derived vesicles to be manufactured stably, economically, and in large quantities through a simplified process.