Acellular Dermal Matrix Cryoprotectant Optimization
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Solution Overview
Problem
Conventional methods for preparing acellular dermal matrices face issues with tissue stability and biological property changes due to inadequate penetration of cryoprotectants and ice crystal formation, leading to rapid degradation and weakened tissue structure.
Innovation Solution
A method involving the removal of epidermis and cells from allograft skin, followed by the preparation of a cryoprotectant solution with glycerol, propylene glycol, and sucrose, which is then penetrating into the skin and freeze-dried to create a stable acellular dermal matrix, optimizing the mixing ratio of sucrose, glycerol, and propylene glycol to prevent ice crystal formation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If conventional cryoprotectant methods are used, then the preparation process is simple, but the cryoprotectant does not sufficiently penetrate into collagen tissues and ice crystals form during freezing
Solution Approach 1:
The patent modifies the chemical composition parameters of the cryoprotectant by adding sucrose to the basic glycerol-propylene glycol solution. This parameter change enables sufficient penetration into collagen tissues while preventing ice crystal formation during freezing, thereby maintaining tissue stability without complicating the preparation process
Solution Approach 2:
The patent creates a composite cryoprotectant system by combining multiple substances (glycerol, propylene glycol, and sucrose) with complementary properties. This composite approach allows the cryoprotectant to simultaneously achieve deep tissue penetration and effective ice crystal prevention, resolving the contradiction between manufacturing simplicity and tissue stability
2Ease of manufacture
If low concentration of sugar is used in cryoprotectant, then the preparation is easier, but moisture cannot be sufficiently excluded from collagen tissues leading to ice crystal formation
Solution Approach 1:
The patent optimizes the sugar concentration parameter to a specific range (20-40% sucrose) in the cryoprotectant solution. This parameter optimization achieves sufficient moisture exclusion from collagen tissues to prevent ice crystal formation while maintaining reasonable ease of preparation through a controlled but not excessive concentration level
3Temperature
If ice crystals form during freezing, then the freezing process is simpler, but many pores are created in tissues during drying and collagen tissues are destroyed
Solution Approach 1:
The patent applies preliminary anti-action by incorporating sucrose into the cryoprotectant before freezing. This pre-prepared composition actively prevents ice crystal formation during the freezing process, thereby avoiding the subsequent harmful effects of pore creation and collagen tissue destruction during drying
4Productivity
If conventional cryoprotectant penetration is used, then the process is faster, but tissue stability is not sufficiently increased and biological properties change
Solution Approach 1:
The patent modifies the cryoprotectant composition parameters by adding sucrose, which enhances penetration capability and tissue stabilization. This parameter modification allows for relatively fast preparation while simultaneously achieving sufficient tissue stability and minimizing biological property changes
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method significantly enhances tissue stability, maintains the extracellular matrix and basement membrane integrity, and minimizes biological property changes, resulting in improved grafting success rates and reduced treatment duration.
Implementation Method 1
penetrating the cryoprotectant into the skin from which epidermis and cells in dermis are removed
Implementation Method 2
low concentration of sugar cannot sufficiently exclude moisture from collagen tissues
Implementation Method 3
many ice crystals are formed at the time of freezing. Such ice crystals make many pores in tissues in the course of drying and destroy collagen tissues
Data Source
AI summary
The present invention relates to a method for producing an acellular dermal matrix, and to an acellular dermal matrix produced by same, and more particularly, to a method for producing an acellular dermal matrix, in which sucrose is added to base ingredients consisting of glycerol, propylene glycol, and a base solvent or solution so as to produce a cryoprotectant, the solution is injected into the skin below the epidermis and dermis from which cells have been removed, and freeze-drying is then performed.