Liquid Detergent Protease Stabilization With Peptide Aldehydes

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Solution Overview

Problem

Subtilisin-type proteases in liquid detergents suffer from degradation, affecting the stability of both the proteases and other enzymes, leading to reduced wash performance.

Innovation Solution

Incorporation of peptide aldehydes or ketone derivatives, particularly those with an OH-substituted phenylalanine as the C-terminal residue, as stabilizers for subtilisin-type proteases in liquid compositions, along with N-terminal protection groups, enhances enzyme stability.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If subtilisin is used in liquid detergent compositions, then wash performance is improved, but the subtilisin degrades other enzymes and itself, reducing stability

Engineering Contradiction:

Improvewash performanceVSAvoidenzyme stability

Core Design Contradiction:

ProductivityVSStability of the object's composition

Solution Approach 1:

The patent introduces peptide aldehydes as intermediary stabilizing agents that mediate between the subtilisin enzyme and the detergent formulation. These peptide aldehydes bind to the subtilisin enzyme, forming a stable complex that prevents self-degradation and protects other enzymes from proteolytic degradation, thereby maintaining enzyme stability while preserving wash performance

Inventive Principle:

Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent modifies the chemical parameters of the detergent formulation by incorporating specific peptide aldehyde compounds with defined amino acid sequences and structural features. This parameter change in the chemical composition enables the stabilizing effect on subtilisin, transforming the system to achieve both high wash performance and enhanced enzyme stability

Inventive Principle:

Principle #35Parameter changes

2Stability of the object's composition

If peptide aldehydes are used as stabilizers, then enzyme stability is improved, but the complexity of the composition increases

Engineering Contradiction:

Improveenzyme stabilityVSAvoidcomposition complexity

Core Design Contradiction:

Stability of the object's compositionVSDevice complexity

Solution Approach 1:

The patent optimizes the concentration parameters of the peptide aldehyde stabilizers to effective levels that provide maximum stabilization with minimal added complexity. By carefully controlling the amount of stabilizer added, the formulation achieves enzyme stability enhancement without excessive complexity in the composition

Inventive Principle:

Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The peptide compounds significantly improve the storage stability of subtilisin and other enzymes, maintaining their activity and enhancing detergency in liquid detergents.

Implementation Method 1

Boric acid and boronic acids are known to reversibly inhibit proteolytic enzymes

Methodology Applied

Scientific EffectReversible inhibition:

Data Source

PatentEP3725797B1Stabilized liquid enzyme compositions

Publication Date: 2026.05.06 NOVOZYMES AS

AI summary

Peptide aldehydes or ketone derivatives are particularly efficient for stabilizing subtilisin-type proteases in aqueous compositions such as liquid detergents, including peptide compounds with OH-substituted phenylalanine aldehyde as the C-terminal residue.