Devascularized Adipose Tissue Scaffold for Soft Tissue Reconstruction
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for soft tissue reconstruction, such as adipose grafting, face limitations including immunogenicity issues with allogeneic tissues and variable treatment outcomes, requiring immunosuppression and frequent re-treatment, and are hindered by the death of adipocytes and replacement with fibrous connective tissue.
Innovation Solution
Development of allogeneic lyophilized and cryopreserved devitalized adipose tissue compositions with low immunogenicity, which can be used for soft tissue reconstruction without the need for immunosuppression or donor-patient matching, and can be administered to treat various soft tissue defects, including congenital and traumatic injuries, using methods that involve subcutaneous injection of these compositions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If allogeneic adipose tissue is used for soft tissue reconstruction, then treatment versatility is improved, but immunogenicity increases causing graft rejection
Solution Approach 1:
The patent extracts and removes the cellular components (adipocytes, stromal cells, immune cells) from the adipose tissue, retaining only the extracellular matrix and structural framework. This decellularization process eliminates the immunogenic cellular elements that cause rejection while preserving the beneficial structural and signaling properties of the tissue for soft tissue reconstruction.
Solution Approach 2:
The patent uses a decellularized intermediate form of adipose tissue that serves as a scaffold or framework. This intermediate structure acts as a mediator between the donor tissue and the patient's body, providing structural support and guiding tissue regeneration without triggering immune rejection, thereby enabling allogeneic use without traditional matching requirements.
2Reliability
If fresh adipose grafts are used, then cell viability is improved, but treatment reliability decreases due to adipocyte death and replacement with fibrous tissue
Solution Approach 1:
The patent applies preliminary processing to the adipose tissue before implantation, including decellularization and stabilization of the extracellular matrix. This preliminary action prepares the tissue in advance to resist degradation and promote stable integration, preventing the premature death of adipocytes and fibrous tissue replacement that occurs with fresh grafts.
Solution Approach 2:
The patent fundamentally changes the physical and chemical parameters of the adipose tissue through decellularization and extracellular matrix stabilization. These parameter changes transform the tissue from a cell-rich, transient structure to a cell-sparse, stable scaffold that maintains its structural integrity and promotes reliable soft tissue reconstruction without the temporal limitations of fresh grafts.
3Object-affected harmful factors
If autologous adipose grafting is used, then immunogenicity is reduced, but device complexity increases due to surgical harvest and grafting procedures
Solution Approach 1:
The patent creates a universal adipose tissue product that can be used across different patients without requiring patient-specific harvesting. The decellularized, stabilized extracellular matrix serves as a universal scaffold that can be implanted in various soft tissue defects across different body sites, eliminating the need for complex surgical harvest and grafting procedures while maintaining low immunogenicity.
4Ease of operation
If conventional adipose grafting is used, then ease of operation is maintained, but productivity decreases due to variable outcomes requiring re-treatment
Solution Approach 1:
The patent employs a disposable, pre-processed decellularized adipose scaffold that is implanted and then allows natural tissue regeneration. This single-use, pre-prepared product eliminates the need for complex surgical harvesting and reduces the need for re-treatment, thereby improving treatment efficiency and productivity while maintaining ease of implantation through simple scaffold placement procedures.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The use of these compositions allows for effective soft tissue reconstruction with reduced immunogenicity and improved treatment outcomes, providing a stable and long-lasting solution for soft tissue defects without the need for frequent re-treatment or immunosuppression, and promotes tissue remodeling and angiogenesis.
Implementation Method 1
allogeneic lyophilized and cryopreserved devitalized adipose tissue compositions
Implementation Method 2
allogeneic lyophilized and cryopreserved devitalized adipose tissue compositions
Implementation Method 3
promotes tissue remodeling and angiogenesis
Data Source
AI summary
Disclosed are devitalized adipose tissue. Disclosed are compositions comprising devitalized adipose tissue. In some aspects, the compositions further comprise a cryopreservation or lyophilization solution. Disclosed are cryopreserved or lyophilized devitalized adipose tissue. Disclosed are methods of augmenting a soft tissue site of a subject in need thereof comprising administering to the subject a composition comprising devitalized adipose tissue. Disclosed are methods of treating a subject having fat pad atrophy comprising administering to the subject a composition comprising devitalized adipose tissue. Disclosed are methods of treating a subject having lipodystrophy comprising administering to the subject a composition comprising devitalized adipose tissue. Disclosed are methods of treating a subject having a metabolic disease or condition comprising administering to the subject a composition comprising devitalized adipose tissue.