Dextran Affinity Tag for Cost-Effective Protein Purification
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Solution Overview
Problem
The high cost of commercial affinity resins used in affinity chromatography for protein purification remains a challenge, necessitating the development of more affordable and effective affinity tags for recombinant protein purification.
Innovation Solution
The introduction of novel affinity tags with specific amino acid sequences, such as those represented by SEQ ID NO: 02 and SEQ ID NO: 10, which exhibit dextran binding ability, allowing for efficient protein purification using dextran-containing resins and washing solutions, thereby reducing production costs.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If commercial affinity resins are used for protein purification, then purification efficiency is improved, but production cost increases
Solution Approach 1:
The patent uses dextran, a inexpensive natural polysaccharide, as the affinity resin material instead of expensive commercial affinity resins. The dextran-based resin provides effective protein purification while significantly reducing production costs, embodying the principle of replacing expensive materials with cheaper alternatives that still fulfill the required function.
Solution Approach 2:
The dextran acts as an intermediary substance that enables protein purification through specific binding interactions. The affinity tag on the target protein interacts with dextran molecules in the resin, allowing for efficient separation and purification without requiring expensive commercial affinity resins.
2Ease of operation
If conventional affinity tags are used, then protein purification is simplified, but resin cost remains high
Solution Approach 1:
The patent changes the fundamental parameter of resin material from expensive commercial affinity resins to inexpensive dextran-based resins. This parameter change maintains the simplicity of affinity purification operations while dramatically reducing resin cost, allowing conventional affinity tag methods to remain easy to operate with much lower material expenses.
3Ease of manufacture
If dextran-based affinity resin is used, then production cost is reduced, but binding specificity must be maintained
Solution Approach 1:
The patent extracts and utilizes the specific binding capability between dextran and its target proteins. By isolating this specific interaction and building the affinity resin around it, the system maintains high binding specificity while using the inexpensive dextran material, thus reducing cost without sacrificing reliability.
Solution Approach 2:
The invention creates a simplified copy of commercial affinity resin functionality using dextran. Instead of using complex, expensive commercial resins, the patent replicates the essential affinity binding function using inexpensive dextran molecules, maintaining specificity while reducing cost.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
These affinity tags enable cost-effective protein purification with high recovery rates, as demonstrated by their ability to bind and elute proteins from dextran-based resins, surpassing the efficiency of conventional His-tag purification methods.
Implementation Method 1
affinity chromatography uses a specific immobilized ligand to specifically interact with the protein itself or the affinity tag
Implementation Method 2
eluting the column with a washing solution to obtain the target protein from the resin; wherein the washing solution contains dextran
Data Source
AI summary
The application relates to a dextran affinity tag and use thereof. The present application provides an affinity tag, which is a segment of dextran binding domain, and can purify a target protein by its affinity with the dextran. The affinity tag of the present application has the advantages of more efficient in preparation and purification, and can be widely used in industrial applications that require protein purification processes.