Dextran and DMSO Cryopreservation Solution for Animal Cells
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Solution Overview
Problem
Current cryopreservation methods for animal cells or tissues in regenerative medicine face challenges in maintaining cell viability and excluding natural components derived from animals, particularly in clinical applications, where solutions like sodium carboxymethylcellulose-based methods are not suitable due to quality and safety concerns at varying storage temperatures.
Innovation Solution
A cryopreservation solution containing specific concentrations of dextran and dimethyl sulfoxide (DMSO) within a physiological aqueous solution, such as Ringer's bicarbonate, which maintains cell viability and excludes natural animal components, ensuring safety and efficacy for clinical use.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If serum or protein components derived from animals are used in cryopreservation, then cell viability is improved, but safety and suitability for clinical regenerative medicine deteriorates
Solution Approach 1:
The invention extracts and removes animal-derived components (serum, protein components) from the cryopreservation solution, replacing them with synthetic alternatives (dextran and DMSO) that maintain cryoprotection efficacy without introducing animal-derived contaminants, thus resolving the contradiction between cell viability and safety for clinical use
Solution Approach 2:
The invention changes the chemical composition parameters of the cryopreservation solution by specifying precise concentrations of dextran (0.5-6.0 w/v%) and DMSO (1.0-15.0 v/v%), transitioning from biological-based formulations to chemically-defined formulations that eliminate animal-derived components while preserving cell viability
2Object-affected harmful factors
If sodium carboxymethylcellulose is used as a cryoprotectant, then animal-derived components are excluded, but clinical applicability deteriorates due to quality and safety concerns
Solution Approach 1:
The invention changes the chemical parameters by replacing sodium carboxymethylcellulose with dextran at optimized concentrations (0.5-6.0 w/v%), improving clinical applicability while maintaining exclusion of animal-derived components
Solution Approach 2:
The invention creates a composite cryopreservation solution combining dextran and DMSO in specific ratios, where dextran provides structural protection and DMSO provides intracellular protection, achieving superior clinical applicability compared to single-component solutions
3Temperature
If conventional cryopreservation methods are used, then freezing capability is achieved, but cell viability and quality at freezing temperature deteriorates
Solution Approach 1:
The invention applies preliminary action by pre-equilibrating cells in the optimized cryopreservation solution containing dextran and DMSO before freezing, allowing cells to adapt to the protective environment and accumulate sufficient cryoprotectant concentration, thereby improving cell viability during the freezing process
Solution Approach 2:
The invention uses a composite cryoprotectant system where dextran forms a protective matrix outside cells and DMSO penetrates inside cells, providing dual-layer protection that maintains cell viability at freezing temperatures better than single-component systems
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution effectively preserves animal cells or tissues by preventing ice crystal formation and maintaining cell viability, allowing for successful freezing and thawing with high recovery rates, making it suitable for clinical applications and long-term storage.
Implementation Method 1
a solution for cryopreservation which includes a cryoprotectant that contains a specific concentration of dextran and DMSO
Implementation Method 2
preserves animal cells or tissues by preventing ice crystal formation and maintaining cell viability
Implementation Method 3
dimethyl sulfoxide; and a physiological aqueous solution
Data Source
AI summary
The present invention provides a solution for cryopreservation of animal cells or animal tissues which substantially includes a cryoprotectant that contains 0.5 w/v % to 6.0 w/v % of dextran or derivatives thereof or salts thereof and contains dimethyl sulfoxide, and includes a physiological aqueous solution; a cryopreserved product of animal cells or animal tissues which includes animal cells or animal tissues, and includes the above-described solution for cryopreservation of animal cells or animal tissues; and a cryopreservation method of animal cells or animal tissues, which is a method using the above-described solution for cryopreservation of animal cells or animal tissues.