DIG-305 Insecticidal Toxin for Coleopteran Pest Control
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Solution Overview
Problem
Current Cry proteins deployed in transgenic plants provide incomplete larval control and are susceptible to resistance development in coleopteran insects, necessitating the development of new Cry proteins with different modes of action to maintain long-term efficacy in pest management.
Innovation Solution
The discovery and use of the DIG-305 insecticidal toxin, which can be used alone or in combination with other Cry proteins, and in conjunction with RNAi methodologies, to control coleopteran and lepidopteran pest populations by targeting specific genes, thereby preventing resistance development.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If current Cry proteins are deployed in transgenic plants, then robust protection against pests is provided, but adult pests emerge indicating incomplete larval control and resistance development
Solution Approach 1:
The patent segments the pest control strategy by combining multiple Cry proteins with different modes of action (Cry1Ab for lepidopteran control, Cry3Bb1 for coleopteran control) into a single transgenic plant event (DAS-59122-7). This segmentation allows each Cry protein to target different pest groups through different mechanisms, preventing any single resistance mechanism from conferring broad resistance and thereby maintaining long-term durability while providing robust protection.
2Device complexity
If single Cry proteins are used for pest control, then simplified management is achieved, but resistance development occurs reducing long-term utility
Solution Approach 1:
The patent merges multiple Cry protein genes (cry1Ab and cry3Bb1) into a single transgenic plant event with coordinated expression. This merging provides simplified pest management through a single event deployment while maintaining durability by combining proteins with different modes of action that target different pest groups, preventing resistance development that would occur with single protein use.
3Reliability
If Cry proteins with same mode of action are used, then consistent control is achieved, but resistance develops through receptor mutations
Solution Approach 1:
The patent applies local quality by assigning different functional properties to different regions of the pest control system: Cry1Ab provides lepidopteran-specific control through one mode of action, while Cry3Bb1 provides coleopteran-specific control through a different mode of action. Each Cry protein maintains consistent control for its target group while the combination provides versatility against multiple pest groups, preventing resistance spread through receptor mutations.
Data Source
AI summary
DIG-305 insecticidal protein toxins, polynucleotides encoding such toxins, and transgenic plants that produce such toxins are disclosed. Also disclosed are methods for using such toxins to control insect pests in plants, especial crop plants. Methods for preparing transgenic plants expressing the protein toxins and methods for detecting the claimed toxins and polynucleotides in transgenic plants are disclosed.