Digoxin Immunochromatographic Test Strip with Fluorescent Microspheres
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Solution Overview
Problem
Current methods for detecting digoxin, such as mass spectrometry, high-performance liquid chromatography, immunoturbidimetry, and chemiluminescence, are costly, require complex sample preparation, have long detection times, and are not suitable for high-throughput or timely monitoring in clinical settings, especially in small hospitals or for individual patient use.
Innovation Solution
An immunochromatographic test strip with a digoxin-specific antibody-fluorescent microsphere complex on a glass fiber mat and a nitrocellulose membrane, allowing for quick, high-throughput detection of digoxin in serum, plasma, or whole blood without the need for refrigeration, with a low detection cost and single-serving packaging.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If mass spectrometry or high-performance liquid chromatography is used for digoxin detection, then measurement precision is improved, but device complexity and cost increase, and productivity decreases due to long detection time and requirement for complex sample preparation
Solution Approach 1:
The patent replaces complex mechanical instrumentation systems (mass spectrometry, high-performance liquid chromatography) with a simplified immunochromatographic test strip system that uses antibody-antigen recognition and capillary action for detection, eliminating the need for complex sample preparation and instrumentation while maintaining detection accuracy
Solution Approach 2:
The patent employs disposable test strips with pre-coated antibodies and fluorescent microspheres that can be used once and discarded, eliminating the need for expensive, complex instruments and refrigerated storage while enabling rapid, point-of-care detection
2Productivity
If immunoturbidimetry or chemiluminescence methods are used for digoxin detection, then productivity is improved through high-throughput capability, but device complexity and cost increase due to requirement for expensive instruments and refrigerated storage
Solution Approach 1:
The patent replaces expensive instrumentation systems (biochemical analyzers, chemiluminescence instruments) with a simple immunochromatographic test strip that uses capillary action and fluorescent microscopy for detection, eliminating the need for refrigerated storage and complex instruments while maintaining high-throughput capability
Solution Approach 2:
The patent creates a simplified copy of the immunoturbidimetry principle using fluorescent microspheres instead of turbidimetric detection, maintaining the high-throughput advantage while eliminating the need for expensive instruments and refrigerated storage
3Productivity
If enzyme multiplied immunization method is used for digoxin detection, then productivity is improved through simultaneous determination of multiple samples, but device complexity increases due to requirement for automatic biochemical analyzer and cold-chain transportation
Solution Approach 1:
The patent replaces automated biochemical analyzers with manual immunochromatographic test strips that use capillary action for sample migration and fluorescent microscopy for detection, eliminating the need for cold-chain transportation and automated instrumentation while maintaining the ability to process multiple samples
Solution Approach 2:
The patent uses disposable test strips instead of reusable automated analyzers, eliminating the need for cold-chain storage and complex instrumentation while enabling rapid, point-of-care detection of multiple samples through parallel testing
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The immunochromatographic test strip provides rapid, accurate, and stable digoxin detection with high sensitivity and repeatability, overcoming the limitations of existing methods by reducing costs, simplifying sample preparation, and enabling timely monitoring at room temperature with single-serving packaging.
Implementation Method 1
the glass fiber mat is sprayed with a digoxin-specific antibody-fluorescent microsphere complex
Implementation Method 2
the nitrocellulose membrane is sequentially provided with a detection line coated with digoxin protein conjugate
Implementation Method 3
a sample pad, a glass fiber mat, a nitrocellulose membrane and a water absorbing paper which are sequentially overlapped on the bottom plate
Data Source
Figure 1~2
AI summary
The present invention provides an immunochromatographic test strip for detecting digoxin and a use thereof, and relates to the field of immunodetection technology. The immunochromatographic test strip for detecting digoxin provided by the present invention includes a bottom plate, and a sample pad, a glass fiber mat, a nitrocellulose membrane and a water absorbing paper which are sequentially overlapped on the bottom plate; the glass fiber mat is sprayed with a digoxin-specific antibody-fluorescent microsphere complex; and the nitrocellulose membrane is sequentially provided with a detection line coated with digoxin protein conjugate and a quality control line coated with a secondary antibody; the digoxin-specific antibody-fluorescent microsphere complex is obtained by conjugation between a digoxin monoclonal antibody or a digoxin polyclonal antibody and a fluorescent microsphere; and the digoxin protein conjugate includes a digoxin-conjugated rabbit albumin or a digoxin-conjugated rabbit ovalbumin. The test strip provided by the present invention has a low cost, is simple and rapid in operation, can be stored and transported at room temperature, has a wide variety of test samples, and has excellent stability and high sensitivity.