Dihydromyricetin extraction and purification process
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Solution Overview
Problem
Existing dihydromyricetin extraction methods face challenges in high production costs due to complex processes, safety risks from high-temperature centrifugation, and introduction of impurities during ultrasonic oscillation, making industrialization difficult.
Innovation Solution
A process using acetone as an extraction solvent for reflux extraction, followed by crystallization with water and decolorization with activated carbon, minimizing energy consumption and reducing the need for costly materials, while ensuring high extraction rates and purity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If ultrasonic-assisted extraction method is used, then extraction efficiency is improved, but production cost increases and decolorization becomes difficult
Solution Approach 1:
The patent changes the extraction parameter from ultrasonic assistance to simple reflux extraction, and adjusts the solvent system to a mixed solvent containing acetone, ethanol, and water in specific proportions (acetone:ethanol:water = 1:(3-5):(8-12)). This parameter change maintains high extraction efficiency while avoiding the high equipment costs and operational complexity of ultrasonic extraction, thereby reducing production costs and improving decolorization ease.
2Manufacturing precision
If high-temperature centrifugation is used, then purification is achieved, but safety risks increase and impurities are introduced
Solution Approach 1:
The patent performs preliminary filtration of the extraction solution before concentration and crystallization steps. This preliminary action removes large particles and impurities early in the process, eliminating the need for subsequent high-temperature centrifugation to achieve purification. The process achieves the desired purification effect through gentle filtration and controlled crystallization, avoiding safety risks associated with high-temperature centrifugation while preventing introduction of additional impurities.
3Manufacturing precision
If complex purification processes are used, then product purity is improved, but process complexity and cost increase
Solution Approach 1:
The patent selectively extracts dihydromyricetin from the plant material using a specifically formulated mixed solvent system. The extraction process is designed to selectively dissolve the target compound while leaving impurities behind. Following extraction, simple filtration and controlled crystallization steps isolate the pure product. This approach achieves high product purity through selective extraction rather than through multiple complex purification steps, thereby reducing process complexity.
4Ease of operation
If conventional extraction solvents are used, then extraction is simple, but energy consumption increases
Solution Approach 1:
The patent modifies the conventional extraction approach by using a mixed solvent system with acetone as a key component, which has higher solubility for dihydromyricetin compared to conventional single solvents. This allows extraction to proceed efficiently at lower temperatures through reflux, reducing the energy input required. The mixed solvent system enhances extraction efficiency, enabling simpler operation with reduced energy consumption compared to conventional high-temperature extraction methods.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The process achieves high dihydromyricetin content (>96%) with reduced costs and simplified operations, suitable for industrial production, and avoids the introduction of impurities, thus enhancing efficiency and safety.
Implementation Method 1
adding an extraction solvent in an amount 5 times that of the raw material to perform reflux extraction
Implementation Method 2
acetone can be used to efficiently extract chlorophyll at room temperature through oscillation
Implementation Method 3
adding water to the extract in proportion and resting for crystallization for 24 hours
Implementation Method 4
adding 5%-10% activated carbon for decolorization and impurity removing
Implementation Method 5
performing suction filtration to obtain light-green sediment underneath
Data Source
AI summary
The present invention relates to the field of dihydromyricetin extraction and purification, and in particular to a dihydromyricetin extraction and purification process. Technical problem: the dihydromyricetin extraction and purification process aims to resolve the technical problems of increased cost of subsequent impurity removing and safety risks in high-temperature centrifugation in the prior art. Technical solution: a dihydromyricetin extraction and purification process: step 1: weighing a raw material, adding an extraction solvent in an amount 5 times that of the raw material to perform reflux extraction, concentrating the filtrate to an extract, and recovering acetone; step 2: resting for crystallization for 24 hours; step 3: performing suction filtration to obtain light-green sediment underneath, and dry the sediment; step 4: adding 5%-10% activated carbon for decolorization and impurity removing; step 5: performing suction filtration; step 6: drying to obtain white powder of dihydromyricetin; and step 7: detecting a content with HPLC.