Dill Aerial Parts Extraction for Small RNA Cosmetics
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Solution Overview
Problem
Current extraction methods for dill (Anethum graveolens) primarily use organic solvents and focus on seeds, with limited research on aerial parts and few cosmetic applications, lacking effective anti-aging products using natural ingredients enriched with low molecular weight RNAs.
Innovation Solution
A method involving solubilizing dill aerial parts in water, adding tetrasodium EDTA to disrupt cell membranes, adjusting pH, and purifying to obtain an aqueous extract enriched with small RNAs of up to 150 nucleotides, which is then filtered and diluted for cosmetic use.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional extraction methods using organic solvents are used, then extraction efficiency is improved, but harmful factors are introduced into the cosmetic product
Solution Approach 1:
The patent changes the extraction parameter from organic solvents to aqueous solution with controlled pH (adjusted to alkaline range using NaOH), maintaining extraction efficiency while eliminating harmful chemical residues in the final cosmetic product
Solution Approach 2:
The patent uses water as the extraction solvent instead of expensive and harmful organic solvents, making the process more environmentally friendly and suitable for cosmetic applications while still achieving effective extraction of bioactive compounds
2Manufacturing precision
If conventional RNA extraction protocols are used, then RNA purification is improved, but other beneficial molecules are removed from the extract
Solution Approach 1:
The patent selectively extracts only small RNAs (through controlled precipitation) while deliberately retaining other beneficial cosmetic molecules such as proteins, sugars, amino acids, and phenolic compounds in the final extract, rather than purifying only RNA
Solution Approach 2:
The patent creates a composite extract containing multiple beneficial components (small RNAs, proteins, sugars, amino acids, phenolic compounds) working together for cosmetic benefits, rather than isolating a single purified component
3Quantity of substance
If extraction focuses on dill seeds, then essential oil yield is improved, but aerial parts bioactive compounds are underutilized
Solution Approach 1:
Instead of focusing on the traditional seed portion of dill for essential oil extraction, the patent inverts the approach by extracting bioactive compounds from the aerial parts (leaves and stems), opening new cosmetic applications for this previously underutilized plant material
4Measurement precision
If small scale laboratory extraction is used, then RNA extraction precision is improved, but production cost and complexity increase for cosmetic application
Solution Approach 1:
The patent simplifies the extraction process by using straightforward parameter adjustments (pH control, temperature, precipitation) rather than complex laboratory protocols, making the process scalable for cosmetic production while maintaining extraction precision
Solution Approach 2:
The extraction process utilizes natural properties of the plant material and simple chemical additions (NaOH for pH adjustment, isopropanol for precipitation) that allow the system to self-organize and separate components without complex equipment or multiple processing steps
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method effectively enriches the extract with small RNAs, proteins, sugars, amino acids, and phenol compounds, providing a natural anti-aging cosmetic ingredient that improves skin and hair health without using harmful chemicals.
Implementation Method 1
adding tetrasodium EDTA to disrupt cell membranes
Implementation Method 2
purifying the mixture obtained by separating the soluble material from the insoluble material
Data Source
AI summary
The invention relates to a method for obtaining an aqueous extract of Anethum graveolens, enriched with small RNAs having a maximum length of 150 nucleotides, from a plant material, said method comprising the following steps: a) aerial parts of dill is brought into contact with water; b) tetrasodium ethylenediaminetetraacetic acid (EDTA) is added at a pH of between 10.5 and 11; c) the pH of the mixture obtained in b) is then adjusted to a value of between 6 and 8; d) the mixture obtained in c) is purified; and e) the pH is checked and readjusted if necessary to a value of between 6 and 8. The invention also relates to an aqueous extract of Anethum graveolens, enriched with small RNAs having a maximum length of 150 nucleotides, obtained by the method.