Diol-Bonded Organic Polymer Chromatography Column
Solution Overview
Problem
Columns packed with silica-based materials cannot withstand alkaline solutions, limiting their washing and sterilization capabilities, which is crucial for maintaining separation performance and removing adsorbed impurities in oligonucleotide separation and analysis.
Innovation Solution
A liquid chromatography method using a packing material formed by fixing a diol structure to crosslinked organic polymer particles, such as crosslinked polyvinyl alcohol, which allows for alkaline washing and sterilization, enhancing separation sensitivity and fractionation of oligonucleotides.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a silica-based packing material is used for oligonucleotide separation, then separation performance is improved, but the column cannot withstand alkaline solutions for washing and sterilization
Solution Approach 1:
The invention uses a composite packing material consisting of a porous organic polymer base material combined with a diol bonding material. This composite structure provides both the separation performance needed for oligonucleotide analysis and the chemical stability required to withstand alkaline washing solutions, eliminating the limitation of silica-based materials that dissolve in base.
2Measurement precision
If silica-based columns are used, then high-sensitivity oligonucleotide separation is achieved, but column washing and sterilization with strong alkali is not possible
Solution Approach 1:
The diol-bonded porous organic polymer composite provides a surface chemistry that enables both high-sensitivity oligonucleotide separation and robust alkaline washing capability, allowing routine column maintenance and sterilization that is not possible with silica-based columns.
3Productivity
If the column is continuously used without alkaline washing, then separation performance degrades due to adsorption of proteins and oligonucleotides, but switching to alkaline-washable columns is not possible with silica-based materials
Solution Approach 1:
The diol-bonded porous organic polymer composite material provides a chemically stable surface that resists adsorption of proteins and oligonucleotides, maintaining consistent separation performance over continuous use and enabling periodic alkaline washing to restore column performance without degradation.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method enables higher sensitivity in oligonucleotide separation and analysis, facilitates column washing and sterilization with alkaline solutions, and improves the fractionation of oligonucleotides, addressing the limitations of silica-based columns.
Implementation Method 1
A method based on hydrophilic interaction chromatography (HILIC) can be performed with an eluent containing a volatile salt at a low concentration
Implementation Method 2
a packing material obtained by fixing a diol to a surface of each of porous particles formed of a crosslinked organic polymer or silica
Data Source
Figure 1
Figure 2
AI summary
The present invention relates to a method of separating and analyzing a mixture of oligonucleotides, including performing liquid chromatography using a column packed with a packing material obtained by fixing a diol to a surface of each of porous particles formed of a crosslinked organic polymer. According to this method, the oligonucleotides can be separated and analyzed with higher sensitivity compared to cases where columns having silica gel as a base material are used. In addition, the column can be washed with an alkaline solution.