Dipeptide-Synthesizing Enzyme Substrate Specificity
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Solution Overview
Problem
Current methods for synthesizing dipeptides, such as chemical and enzymatic synthesis, face inefficiencies and environmental concerns, and existing dipeptide-synthesizing enzymes like bacilysin synthetase have limited substrate specificity, making it difficult to produce all dipeptides efficiently.
Innovation Solution
A protein with dipeptide-synthesizing activity is produced using a microorganism transformed with recombinant DNA encoding a protein sequence with specific amino acid modifications, allowing for the formation of dipeptides by peptide bond formation at the α-carboxyl group of L-amino acids, enabling the production of various dipeptides.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If chemical synthesis methods are used for dipeptide production, then dipeptides can be synthesized, but operations such as introduction and removal of protective groups are necessary and racemates are formed, causing difficulties in efficiency and environmental hygiene
Solution Approach 1:
The patent replaces chemical synthesis methods with an enzymatic synthesis method using a novel dipeptide-synthesizing enzyme. This substitution eliminates the need for protective groups and harsh chemical reagents, thereby improving efficiency and reducing environmental pollution while maintaining the ability to synthesize dipeptides.
Solution Approach 2:
The invention changes the fundamental reaction parameters by using an enzyme-catalyzed reaction instead of chemical synthesis. This allows the reaction to proceed under milder conditions without requiring protective groups, reducing waste and improving atom economy while achieving high dipeptide synthesis efficiency.
2Productivity
If existing dipeptide-synthesizing enzymes like bacilysin synthetase are used, then dipeptide formation is possible, but substrate specificity is limited, making it difficult to produce all dipeptides efficiently
Solution Approach 1:
The patent describes a novel dipeptide-synthesizing enzyme that can catalyze the formation of various dipeptides from different combinations of amino acids. This universal enzyme can accept multiple substrates and produce diverse dipeptide products, overcoming the limited substrate specificity of existing enzymes like bacilysin synthetase while maintaining high catalytic efficiency.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables the efficient production of dipeptides with diverse substrate specificity, overcoming the limitations of existing methods by using a protein expressed in a transformant or microorganism to form dipeptides from different combinations of amino acids.
Implementation Method 1
a protein having dipeptide-synthesizing activity... allowing the dipeptide to form and accumulate in the medium
Data Source
AI summary
The present invention provides: a protein having dipeptide-synthesizing activity; DNA encoding the protein; a recombinant DNA comprising the DNA; a transformant transformed with the recombinant DNA; a process for producing the protein having dipeptide-synthesizing activity using the transformant or the like; a process for producing a dipeptide using the protein having dipeptide-synthesizing activity; and a process for producing a dipeptide using, as an enzyme source, a culture of a transformant or a microorganism which produces the protein having dipeptide-synthesizing activity or the like.