DMSO-Free Cell Preservation Composition for Broad-Temperature Storage
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Solution Overview
Problem
Current cell preservation solutions require specific hypothermic preservation temperatures (2° C.-8° C.) and often use DMSO, which are physiologically toxic, leading to poor preservation efficiency, low cell survival rates, and difficulty in mass production, especially for autologous and allogeneic cell preparations.
Innovation Solution
A cell preservation solution composition comprising a copolymer of compound A (60.00 wt % to 99.5 wt %) and compound B (0.05 wt % to 40.00 wt %), allowing preservation at temperatures ranging from hypothermic to room temperature (2° C.-37° C.), without DMSO, and including additives to reduce cell damage.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If DMSO is used as cryoprotectant, then cell preservation effectiveness is improved, but physiological toxicity increases causing hypertension, nausea, and vomiting
Solution Approach 1:
The patent removes DMSO from the preservation solution formulation. The solution uses alternative cryoprotectants (polyethylene glycol, glycerin, sucrose) to achieve cell preservation without the toxic effects of DMSO, thereby extracting the harmful component while maintaining the beneficial preservation function.
Solution Approach 2:
The patent employs a composite preservation solution containing multiple components (polyethylene glycol, glycerin, sucrose, and other additives) that work synergistically to protect cells during freezing and thawing, replacing the single-component DMSO approach with a multi-component system that achieves both preservation effectiveness and reduced toxicity.
2Reliability
If hypothermic preservation temperature (2° C.-8° C.) is maintained, then cell preservation is achieved, but operational complexity and cost increase due to continuous temperature control requirements
Solution Approach 1:
The patent modifies the chemical composition parameters of the preservation solution by incorporating specific concentrations of polyethylene glycol, glycerin, and sucrose, which alter the solution's freezing point and thermal properties. This enables the solution to maintain cell viability across a broader temperature range, reducing the stringency of temperature control requirements.
Solution Approach 2:
The preservation solution is designed to function effectively across multiple temperature conditions (hypothermic, refrigerated, and even room temperature), making it universally applicable without requiring specialized temperature control equipment or procedures for each storage condition.
3Duration of action of stationary object
If current preservation solutions are used, then cell storage is possible, but preservation effectiveness is poor leading to low cell survival rate and short effective preservation time
Solution Approach 1:
The patent uses a composite formulation combining polyethylene glycol (for cryoprotection and viscosity control), glycerin (for osmotic balance and membrane protection), and sucrose (for metabolic support and structural stability). This multi-component system provides synergistic protection that extends effective preservation time while maintaining high cell survival rates.
Solution Approach 2:
The preservation solution acts as an intermediary medium that mediates between the cell and the harsh freezing environment. The specific combination of cryoprotectants and additives in the solution provides gradual osmotic adjustment and metabolic support during the freezing and thawing processes, thereby extending viable preservation duration.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enhances cell storage time and survival rate, enabling effective preservation without the need for pre-cooling and maintaining low temperatures, and supports cell functionality post-thawing.
Implementation Method 1
The cell preservation solution composition excludes dimethyl sulfoxide (DMSO) and includes a copolymer containing compound A and compound B... enhancing cell storage time and survival rate, enabling effective preservation without the need for pre-cooling and maintaining low temperatures
Implementation Method 2
During the freezing process, the formation of intracellular ice crystals and the imbalance of osmotic pressure may damage cells. In order to avoid the above situation, cryoprotectant (CPA) are used to freeze cells.
Data Source
AI summary
A cell preservation solution composition, excluding dimethyl sulfoxide (DMSO), includes a copolymer of compound A and compound B. In a composition of 100 wt % of the cell preservation solution composition, the compound A ranges from 60.00 wt % to 99.95 wt %, and compound B ranges from 0.05 wt % to 40.00 wt %.