Multivalent DNA Antibody Constructs for Drug-Resistant Gonorrhea
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Solution Overview
Problem
There is a need for improved therapeutics to combat multi-drug resistant Neisseria gonorrhoeae infections, as current treatments are limited and ineffective against this pathogen.
Innovation Solution
The development of recombinant nucleic acid sequences encoding synthetic antibodies, such as multivalent antibodies, which can be administered to induce in vivo expression of antibodies capable of binding and neutralizing Neisseria gonorrhoeae antigens, thereby inducing an immune response and providing therapeutic benefits.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If current treatment regimens are used, then treatment simplicity is maintained, but effectiveness against multi-drug resistant Neisseria gonorrhoeae is insufficient
Solution Approach 1:
The antibody is divided into multiple functional domains (variable regions for antigen binding, constant regions for immune effector functions) that can be independently optimized and recombined to create multivalent constructs with enhanced effectiveness against resistant strains
Solution Approach 2:
The patent creates composite antibody structures by combining multiple antibody fragments and domains (e.g., scFv, Fc regions, hinge regions) into multivalent constructs that integrate both antigen-binding functionality and immune-modulating properties to overcome drug resistance
2Reliability
If synthetic antibodies are administered, then rapid protection and survival are achieved, but the complexity of antibody construction increases
Solution Approach 1:
The antibody constructs are pre-engineered with optimized variable regions, constant regions, and multivalent configurations before administration, allowing them to immediately bind and neutralize antigens upon delivery without requiring further assembly or activation in the host
Solution Approach 2:
The multivalent antibody constructs are designed to perform multiple functions simultaneously: binding to multiple antigen epitopes for enhanced neutralization, engaging Fc receptors for immune cell activation, and providing extended half-life through FcRn binding, thereby achieving rapid protection through a single therapeutic agent
Data Source
AI summary
Disclosed herein are compositions for generating a synthetic antibody or synthetic multivalent antibody in a subject. Also disclosed are methods for generating a synthetic antibody or synthetic multivalent antibody in a subject by administering a composition including one or more recombinant nucleic acid sequence that encodes a synthetic antibody or synthetic multivalent antibody to the subject. The disclosure also provides a compositions and methods of preventing and/or treating a Neisseria gonorrhoeae infection in a subject using said synthetic antibody or synthetic multivalent antibody.