DNA Aptamer Stabilization via Mini-Hairpin Substitution
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Solution Overview
Problem
Current methods for enhancing the stability and target-binding capacity of DNA aptamers are costly and inefficient, as chemical modifications often compromise structural integrity and binding affinity, and are difficult to implement on a large scale.
Innovation Solution
Substituting internal hairpin structures with mini-hairpin structures and optionally replacing A-T base pairs with G-C base pairs in the stem structure of DNA aptamers to improve stability and binding capacity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stability of the object's composition
If chemical modifications are made to sugar or phosphate portions to improve stability, then stability against nucleolytic enzymes is improved, but binding capacity to target molecule deteriorates and production cost increases
Solution Approach 1:
The invention changes the nucleotide sequence parameters (substituting pyrimidine bases with purine bases, adjusting GC content) rather than chemically modifying the backbone, thereby improving stability while preserving binding capacity and avoiding increased production costs
2Stability of the object's composition
If chemical modifications are made to enhance stability, then stability is improved, but production cost increases
Solution Approach 1:
The invention uses nucleotide sequence parameter changes (base substitutions) that can be implemented through standard DNA synthesis methods, avoiding costly chemical modification processes while achieving enhanced stability
3Stability of the object's composition
If chemical modifications are made to improve stability, then stability is improved, but structural integrity deteriorates
Solution Approach 1:
The invention modifies nucleotide sequence parameters (base composition and GC content) while maintaining the overall hairpin structural framework, thereby improving stability without compromising the higher-order structure required for function
Data Source
AI summary
An object of the present invention is to provide a convenient and low-cost method for enhancing the stability of a DNA aptamer and/or its capacity to bind to a target molecule, and a DNA aptamer obtained by the method. The object is solved by substituting an internal hairpin structure (stem-loop structure) of the DNA aptamer with a structure called mini-hairpin structure and optionally increasing GC pairs in a stem portion of the DNA aptamer.