DNA Elongation via Bound Factor Removal
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Solution Overview
Problem
Current methods for elongating DNA are limited in effectively removing bound factors, which constrain DNA orientation and length, hindering sequence analysis and processing.
Innovation Solution
The method involves immobilizing DNA to a substrate and using reagents or forces to separate and remove bound factors, allowing the DNA to achieve its natural elongated state based on its nucleic acid sequence, enabling further processing such as fluorescence in situ hybridization, sequencing, or nanopore sequencing.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If DNA is kept in its natural state with bound factors, then the DNA maintains its biological function and structure, but the DNA cannot be effectively elongated for sequence analysis
Solution Approach 1:
The patent extracts bound factors (proteins, RNA, metabolites) from the DNA complex through enzymatic treatment and chemical processing, separating the DNA from components that prevent elongation. This extraction allows the DNA to achieve its full extended length while maintaining the DNA backbone integrity for subsequent analysis.
2Ease of operation
If bound factors are removed from DNA to enable elongation, then the DNA can be analyzed, but the natural state and orientation information is lost
Solution Approach 1:
The patent performs preliminary actions by first isolating and characterizing the bound factors before removing them, and by capturing orientation data through imaging and mapping techniques before elongation. This preliminary characterization preserves information about the natural state while enabling subsequent elongation and analysis.
3Length of moving object
If DNA is elongated by removing bound factors, then the DNA length increases for better sequencing, but the time required for factor removal increases
Solution Approach 1:
The patent employs parameter changes by optimizing enzymatic reaction conditions (temperature, pH, enzyme concentration) and using multiple parallel processing steps to accelerate bound factor removal. These parameter optimizations reduce the time required for elongation while achieving complete factor removal for high-quality sequencing.
Data Source
AI summary
The present invention relates to methods of elongating chromosomes. Embodiments of the present disclosure are directed to methods of elongating DNA by immobilizing or attaching the DNA to a substrate. According to one aspect, naturally occurring DNA includes a nucleic acid and one or more factors bound thereto, and may be referred to herein as “starting DNA”.