DNA Microarray Probes for Acute Enteritis Virus Detection
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Solution Overview
Problem
Current methods for diagnosing acute enteritis viruses such as norovirus, rotavirus, hepatitis A virus, coxsackievirus, astrovirus, and adenovirus are costly, require specialized equipment, and have long trial periods, making rapid and accurate detection challenging.
Innovation Solution
A composition including specific probes for each virus type, a DNA microarray, and a detection kit that uses hybridization reactions to identify these viruses, allowing for simultaneous detection without expensive equipment or specialists.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional diagnostic methods are used for acute enteritis viruses, then detection accuracy can be maintained, but detection cost increases and detection time extends
Solution Approach 1:
The invention divides the detection system into multiple specific probe segments, each targeting a particular virus type (norovirus, rotavirus, hepatitis A, coxsackievirus, astrovirus, adenovirus). This segmentation allows parallel detection of multiple viruses simultaneously, reducing total detection time while maintaining accuracy for each virus type through specialized probe design
Solution Approach 2:
The DNA microarray platform serves as a universal detection system that can detect multiple different virus types using a single platform. The microarray integrates multiple probe functions into one device, enabling simultaneous detection of six different enteric viruses without requiring separate diagnostic equipment for each virus type
2Reliability
If conventional diagnostic methods are used for acute enteritis viruses, then reliable detection can be achieved, but equipment complexity and operational difficulty increase
Solution Approach 1:
The invention employs a disposable DNA microarray kit that can be used once and then discarded, eliminating the need for expensive, complex, and maintenance-intensive equipment. The microarray itself acts as a single-use detection device that provides reliable results without requiring sophisticated instrumentation or specialized operational procedures
Solution Approach 2:
The detection system is designed to be self-explanatory and self-executing to the extent possible, with probes that automatically hybridize with target viral nucleic acids under standardized conditions. The system minimizes the need for operator expertise by using pre-designed probes and standardized protocols that reduce human intervention and interpretation complexity
3Adaptability or versatility
If conventional diagnostic methods are used for acute enteritis viruses, then comprehensive virus identification is possible, but operational cost increases
Solution Approach 1:
The invention merges the detection capabilities for six different virus types into a single integrated DNA microarray platform. By combining multiple detection functions into one device and one test procedure, the system achieves comprehensive virus identification coverage while reducing the cumulative cost of performing separate tests for each virus type
Solution Approach 2:
The invention changes the fundamental detection parameter from expensive conventional methods (such as cell culture or complex molecular biology techniques) to a more cost-effective nucleic acid hybridization approach. This parameter change maintains the ability to identify multiple virus types while significantly reducing per-test costs through the use of synthesized DNA probes and simplified detection chemistry
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution enables rapid, accurate, and cost-effective detection of acute enteritis viruses through specific and sensitive hybridization reactions, facilitating early diagnosis and potential vaccine development.
Implementation Method 1
The present invention relates to a composition including a probe for detecting six representative types of viruses causing acute enteritis
Data Source
AI summary
The present invention relates to a composition comprising a probe for detecting six representative causative viruses of acute enteritis (norovirus genogroup I and genogroup II, rotavirus, hepatitis A virus, coxsackievirus, astrovirus, and adenovirus), and a DNA microarray, a kit, and a detection method comprising the composition. The present invention is effective due to high specificity and sensitivity to viruses. In addition, since the causative viruses can simply be detected at low cost compared to conventional detection methods, without expensive diagnosis devices or specialists, the present invention may be effectively used as a method for diagnosing viruses causing acute enteritis.