DNA Walker Aflatoxin B1 Detection Kit
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for detecting aflatoxin B1 (AFB1) are costly, time-consuming, and require specialized equipment and skilled labor, with traditional ELISA techniques being prone to enzyme denaturation and having long culture times, necessitating a rapid and cost-effective detection method.
Innovation Solution
A detection kit utilizing a DNA Walker structure and hyperbranched fluorescent nanotree structure, incorporating aptamers and endonucleases, which enables dual signal amplification for enhanced sensitivity and speed in AFB1 detection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional HPLC or GC/MS methods are used for AFB1 detection, then detection accuracy is improved, but instrument cost and operational complexity increase significantly
Solution Approach 1:
The patent replaces complex instrumental analysis systems (HPLC, GC/MS) with a nucleic acid-based detection system that uses fluorescent signals and enzyme reactions. The detection mechanism substitutes mechanical/chromatographic separation with molecular recognition through aptamers and DNA walkers, eliminating the need for expensive instruments while maintaining detection capability.
Solution Approach 2:
The patent creates a simplified detection model by using aptamer-DNA walker complexes that replicate the separation and detection functions of HPLC/GC/MS at a much simpler level. The DNA walker structure copies the analytical separation process through enzymatic steps, producing detectable signals without requiring complex instrumentation.
2Measurement precision
If traditional ELISA methods are used for AFB1 detection, then detection capability is achieved, but test time and enzyme stability issues worsen
Solution Approach 1:
The patent changes the fundamental parameters of the detection system by replacing enzyme-based ELISA with nucleic acid-based detection. The detection mechanism uses aptamer binding and DNA walker enzymatic steps that complete in minutes rather than hours or days, dramatically reducing test time while maintaining sensitivity through fluorescent signal amplification.
Solution Approach 2:
The patent extracts and eliminates the time-consuming culture and elution steps from traditional ELISA procedures. By using pre-formed aptamer-DNA walker complexes that bind AFB1 directly without requiring microbial culture, the method removes the source of time loss while maintaining detection capability through direct molecular recognition.
3Measurement precision
If traditional ELISA methods are used for AFB1 detection, then detection is achieved, but enzyme denaturation and inactivation increase
Solution Approach 1:
The patent replaces expensive, unstable enzymes with stable nucleic acid molecules (aptamers and DNA walkers) that can be stored and handled more reliably. The detection system uses synthetic oligonucleotides that do not denature or invert, eliminating the stability issues inherent in enzyme-based methods while maintaining detection function.
Solution Approach 2:
The patent substitutes enzyme-based detection with nucleic acid-based detection, replacing the unstable protein catalysts with stable molecular recognizers. The aptamer-DNA walker system uses base-pairing and enzymatic cleavage of nucleic acids instead of enzyme catalysis, eliminating denaturation and inactivation problems.
4Productivity
If rapid detection methods are developed, then test speed is improved, but detection sensitivity may deteriorate
Solution Approach 1:
The patent segments the detection process into distinct functional modules: aptamer binding, DNA walker enzymatic steps, and fluorescent signal generation. Each segment can be optimized independently, allowing rapid enzymatic steps to maintain speed while the fluorescent amplification system ensures high sensitivity through signal multiplication.
Solution Approach 2:
The patent adds a fluorescent signal amplification dimension to the detection process. By incorporating fluorescent groups and signal amplification steps, the system achieves high sensitivity not through increased concentration of target analysis but through optical signal multiplication, allowing rapid detection without sacrificing sensitivity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The kit achieves rapid, sensitive, and cost-effective detection of AFB1 with improved reaction speed and specificity, reducing the need for expensive instruments and skilled labor, while maintaining high sensitivity and specificity.
Implementation Method 1
Aptamers are short, single-stranded oligonucleotide sequences (DNA, RNA, or nucleic acid analogs) selected from a nucleic acid molecular library using the in vitro systematic evolution of ligands by exponential enrichment (SELEX) method. Like antibodies, aptamers have strict recognition and high affinity for binding ligands.
Implementation Method 2
The kit of the invention realizes dual signal amplification based on DNA Walker structure and hyperbranched fluorescent nanotree structure. This method not only enhances the detection signal of AFB1 and improves the reaction speed, but also realizes the high sensitive and rapid detection of AFB1.
Implementation Method 3
The sequence E1 on the W chain and the sequence E2 on the S1 chain can form the recognition site of endonuclease by base complementary pairing.
Data Source
AI summary
The invention displays aflatoxin B1 (AFB1) detection kit and AFB1 detection method. The invention belongs to the technical field of detecting harmful substances. The AFB1 detection kit was fabricated with DNA walker structure, endonuclease, hairpin H1 and H2. The AFB1 detection kit has benefits of high sensitivity and short detection time based on signal amplification strategy of DNA Walker structure and hyperbranched fluorescent nanotrees. The present invention can realize high sensitive and rapid detection of AFB1.