Double-Layer Cell Culture Bag for Gas Exchange and Water Retention
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Solution Overview
Problem
Conventional cell culture flasks with large volumes pose challenges in gas exchange and convenience due to their size, limiting the ability to maintain optimal gas conditions for cell growth.
Innovation Solution
A cell culture bag with a plastic bag body and double layer membrane structure, featuring a gas permeable surface and connectors for fluid communication, optimized for oxygen and water vapor transmission rates, and a thickness range of 50 μm to 140 μm to ensure efficient gas exchange and cell survival.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If a gas permeable film is attached to the bottle mouth of the culture flask, then gas exchange is enabled, but the large volume of the culture flask causes inconvenience in use and limits gas exchange efficiency
Solution Approach 1:
The patent employs a flexible plastic bag body with integrated gas permeable surfaces instead of a rigid culture flask with an attached film. The bag can be folded, compressed, and handled easily, while maintaining gas exchange functionality through the permeable membrane material that forms the bag walls themselves.
Solution Approach 2:
The invention divides the culture system into a flexible bag body with connectors for fluid communication, separating the culture function from the gas exchange function. The gas permeable surfaces are distributed across the bag body rather than concentrated at a single bottle mouth, enabling simultaneous cell culture and efficient gas exchange.
2Volume of stationary object
If the culture flask volume is large, then sufficient gas accommodation is achieved, but gas exchange efficiency and convenience deteriorate
Solution Approach 1:
The flexible plastic bag body allows the culture volume to expand or contract as needed while maintaining efficient gas exchange through the permeable surfaces. The bag can be compressed to increase gas contact area without increasing overall volume, optimizing gas exchange efficiency regardless of culture volume.
Solution Approach 2:
The patent transitions from a rigid three-dimensional flask geometry to a flexible two-dimensional bag structure that can be folded and compressed. This dimensional flexibility allows the same culture volume to achieve greater gas exchange surface area by collapsing the bag into a more compact form factor.
3Reliability
If a double layer membrane with optimized thickness is used, then gas permeability is enhanced, but material complexity increases
Solution Approach 1:
The patent uses a double layer membrane structure where the first layer provides structural support and the second layer provides gas permeability. This composite material approach achieves optimized gas permeability while maintaining bag integrity, with the added benefit that the layers can be manufactured separately and bonded together using simple heat sealing processes.
Solution Approach 2:
The invention optimizes the thickness parameters of the double layer membrane to achieve the desired balance between mechanical strength and gas permeability. By carefully controlling the thickness of each layer within specific ranges, the patent achieves high gas permeability without requiring complex multi-layer or heterogeneous structures.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The cell culture bag provides enhanced gas permeability, maintaining optimal gas conditions for cell growth while minimizing water vapor loss, ensuring high cell survival rates and ease of use.
Implementation Method 1
The culture space and an external environment are in gas communication via the gas permeable surface
Implementation Method 2
an edge sealing section formed by heat sealing the second layer
Implementation Method 3
An oxygen transmission rate of the cell culture bag is higher than 200 g/(m2·day), and a water vapor transmission rate of the cell culture bag is lower than 10 g/(m2·day) measured at an environment of 37° C. and 1 atm
Implementation Method 4
An oxygen transmission rate of the cell culture bag is higher than 200 g/(m2·day), and a water vapor transmission rate of the cell culture bag is lower than 10 g/(m2·day) measured at an environment of 37° C. and 1 atm
Data Source
AI summary
A cell culture bag is provided. The cell culture bag includes a plastic bag body and at least two connectors disposed on the plastic bag body. A culture space and an external environment are in fluid communication by the connectors. The cell culture bag is formed from a double layer membrane, and a material of the double layer membrane is a polyolefin. The double layer membrane includes a first layer and a second layer, and a melting point of the first layer is higher than a melting point of the second layer. The plastic bag body includes a culture space and a gas permeable surface. The culture space is formed in the plastic bag body. The second layer faces toward the culture space. The culture space and an external environment are in gas communication via the gas permeable surface.