Doubled-Haploid Corn Breeding via Chromosome Doubling
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
The development of new inbred corn plants is a slow and costly process that requires expertise, and existing inbred corn lines lose commercial competitiveness over time, necessitating the creation of improved germplasm with enhanced yield, disease resistance, and adaptability.
Innovation Solution
The introduction of the inbred corn line 2EEXX1039, which is a yellow dent inbred corn line with specific physiological and morphological characteristics, and methods for producing and breeding new inbred and hybrid corn plants using techniques such as self-pollination, sib-pollination, backcrossing, haploid breeding, and tissue culture to create stable and high-yielding corn varieties.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If traditional inbreeding techniques (self-pollination and sib-pollination) are used to develop new inbred corn lines, then new inbred lines can be obtained, but the process is slow and costly with decreasing vigor after sufficient inbreeding
Solution Approach 1:
The patent applies preliminary action by using chemical treatments (colchicine, oryzalin) on immature embryos or pollen to induce chromosome doubling before the plants reach maturity. This preliminary chromosomal modification allows direct production of homozygous doubled-haploid plants from heterozygous parents, bypassing the need for multiple generations of traditional inbreeding and immediately achieving the stable homozygous state that would otherwise require extensive self-pollination over many generations.
2Quantity of substance
If traditional inbreeding is continued after sufficient inbreeding is achieved, then more seed of the developed inbred is produced, but vigor decreases and additional inbreeding merely increases seed without improvement
Solution Approach 1:
The patent applies preliminary action by performing chromosome doubling on immature embryos or pollen before the plants complete their development. This preliminary genetic modification creates doubled-haploid plants that are immediately homozygous and genetically stable, eliminating the need for continued inbreeding. The resulting plants maintain full vigor while producing the required seed quantity, as the homozygous state is achieved in one generation rather than through prolonged inbreeding that depletes genetic vigor.
3Duration of action of stationary object
If existing inbred corn lines are used over time, then production is maintained, but they lose commercial competitiveness due to lack of enhanced yield and disease resistance
Solution Approach 1:
The patent applies parameter changes by fundamentally altering the genetic composition parameter of corn lines through chromosome doubling to create doubled-haploid plants. This parameter change enables the rapid generation of completely homozygous lines with novel genetic combinations that exhibit enhanced yield, disease resistance, and adaptability. The technique transforms the genetic state from heterozygous to homozygous in one generation, creating new inbred lines with improved commercial competitiveness while extending their useful lifespan.
Data Source
AI summary
An inbred corn line, designated 2EEXX1039, the plants and seeds of the inbred corn line 2EEXX1039, methods for producing a corn plant, either inbred or hybrid, produced by crossing the inbred corn line 2EEXX1039 with itself or with another corn plant, and hybrid corn seeds and plants produced by crossing the inbred line 2EEXX1039 with another corn line or plant and to methods for producing a corn plant containing in its genetic material one or more transgenes and to the transgenic corn plants produced by that method. This invention also relates to inbred corn lines derived from inbred corn line 2EEXX1039, to methods for producing other inbred corn lines derived from inbred corn line 2EEXX1039 and to the inbred corn lines derived by the use of those methods.