DP-915635-4 Corn Event Detection Using Flanking DNA Sequences

Resolve Bottlenecks,
Find Innovative SolutionsGenerate Solutions

Solution Overview

Problem

Existing methods for detecting transgenic insect-resistant corn events, such as DP-915635-4, are inadequate for distinguishing between different events due to interchangeable coding regions and lack of specificity in DNA constructs, making it difficult to identify the presence of specific transgenes in progeny.

Innovation Solution

Development of DNA constructs and detection methods using specific primers and probes that recognize unique 5′ and/or 3′ flanking sequences of DP-915635-4, enabling accurate identification through PCR and hybridization techniques.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If general nucleic acid detection methods (PCR, DNA hybridization) are used to detect transgenes, then the presence of transgenes can be detected, but the ability to distinguish between different transgenic events is lost due to interchangeable coding regions

Engineering Contradiction:

Improvespecificity of transgene detectionVSAvoidevent discrimination capability

Core Design Contradiction:

Measurement precisionVSLoss of information

Solution Approach 1:

The detection method segments the transgenic event into two distinguishable parts: the interchangeable coding region (which cannot be used for differentiation) and the unique flanking DNA sequences (which can be used for differentiation). By targeting the flanking sequences with event-specific primers, the method achieves event discrimination despite the interchangeability of coding regions.

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

The invention applies local quality by focusing the detection specificity on the flanking DNA sequences rather than the coding region. The flanking sequences have unique local characteristics (specific nucleotide sequences) that differ between events, allowing them to serve as distinctive markers for event identification while the coding regions remain interchangeable.

Inventive Principle:

Principle #3Local quality

2Adaptability or versatility

If DNA constructs with interchangeable coding regions are used, then gene function can be maintained, but the ability to identify specific events in progeny is compromised

Engineering Contradiction:

Improveinterchangeability of coding regionsVSAvoidevent identification accuracy

Core Design Contradiction:

Adaptability or versatilityVSMeasurement precision

Solution Approach 1:

The method segments the DNA construct into functional components: interchangeable coding regions that maintain gene function and unique flanking sequences that provide event-specific identification. This segmentation allows the coding regions to be interchangeable while the flanking sequences provide the necessary specificity for event identification in progeny.

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

The flanking DNA sequences act as an intermediary element that bridges the interchangeable coding regions with event-specific identification. These flanking sequences are present in the genome flanking the inserted transgene and serve as mediators that enable event discrimination without affecting the functionality of the coding regions.

Inventive Principle:

Principle #24Intermediary (Mediator)

3Ease of manufacture

If detection methods focus on frequently used genetic elements (promoters, terminators, marker genes), then detection can be performed, but discrimination between different events produced using the same construct is not possible

Engineering Contradiction:

Improvedetection method simplicityVSAvoidevent discrimination capability

Core Design Contradiction:

Ease of manufactureVSMeasurement precision

Solution Approach 1:

Instead of detecting the commonly used but interchangeable genetic elements (promoters, terminators, marker genes) that are present across multiple events, the method inverts the approach by detecting the flanking DNA sequences that are unique to each event. This inversion of the detection target enables event discrimination while maintaining detection simplicity through the use of specific primers against unique flanking sequences.

Inventive Principle:

Principle #13The other way round (Inversion)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Enables precise detection of the DP-915635-4 event in corn plants and progeny, ensuring effective identification and segregation of transgenic material from non-transgenic material.

Implementation Method 1

contacting the sample with a DNA primer set that when used in a nucleic acid amplification reaction with genomic DNA extracted from corn comprising event DP-915635-4 produces an amplicon that is diagnostic for corn event DP-915635-4

Methodology Applied

Scientific EffectPolymerase chain reaction (PCR):

Implementation Method 2

detecting the amplicon

Methodology Applied

Scientific EffectNucleic acid hybridization:

Data Source

PatentUS20260049330A1Maize event DP-915635-4 and methods for detection thereof

Publication Date: 2026.02.19 PIONEER HI BREED INTERNATIONAL INC

AI summary

Embodiments disclosed herein relate to the field of plant molecular biology, specifically to DNA constructs for conferring insect resistance to a plant. Embodiments disclosed herein relate to insect resistant corn plant containing event DP-915635-4, and to assays for detecting the presence of event DP-915635-4 in samples and compositions thereof.