Integrated dPCR Chip Analysis for Faster, Cleaner Detection
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Solution Overview
Problem
Existing digital polymerase chain reaction (dPCR) products require multiple ancillary apparatuses, leading to long detection times, high costs, complex operation steps, and risks of reagent contamination.
Innovation Solution
An integrated analysis device for a detection chip that combines a loading part, temperature control part, and signal detection part, allowing for a single-device operation, reducing ancillary apparatuses, simplifying steps, and minimizing contamination risks.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If multiple ancillary apparatuses are used for dPCR detection, then detection accuracy and functionality are improved, but device complexity and operation complexity increase
Solution Approach 1:
The patent combines multiple independent detection functions (temperature control, optical detection, loading/unloading) into a single integrated apparatus. The detection chip is positioned on a stage that serves as both a loading platform and a temperature control substrate, while optical detectors are integrated above the chip area, eliminating the need for separate ancillary devices and reducing operational complexity while maintaining detection accuracy
Solution Approach 2:
The detection chip stage serves multiple functions simultaneously: it acts as a mechanical support for loading/unloading chips, a thermal substrate for temperature control during PCR cycles, and a positioning platform for optical detection. This multi-functionality reduces the number of separate apparatuses needed while maintaining all necessary detection capabilities
2Reliability
If multiple ancillary apparatuses and complex steps are used, then detection completeness is improved, but detection time and operation complexity increase
Solution Approach 1:
The integrated apparatus allows continuous detection operations without requiring physical transfer of the detection chip between separate devices. The chip remains on the stage throughout the entire process, enabling continuous temperature cycling and continuous optical detection, thereby eliminating idle transfer time while maintaining complete detection functionality
Solution Approach 2:
By merging temperature control and optical detection into a single integrated system, the patent eliminates the sequential operation required when using separate apparatuses. Both functions operate simultaneously on the same chip position, reducing total detection time while ensuring all detection steps are completed
3Reliability
If multiple apparatuses and transfer steps are used, then detection capability is improved, but contamination risk increases
Solution Approach 1:
The integration of all detection functions into a single apparatus eliminates the need to transfer the detection chip between multiple devices. The chip remains sealed and contained throughout the entire detection process, with reagents never exposed to external environments, thereby minimizing contamination risk while maintaining full detection capability
Solution Approach 2:
The detection chip is designed to be self-contained with all necessary reagents and samples sealed within the chip structure. The integrated apparatus interacts with the chip through controlled interfaces (heating from below, optical detection through transparent regions), allowing the chip to perform all detection functions without external intervention that could introduce contamination
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The integrated device reduces detection time, lowers costs, and minimizes reagent contamination by consolidating functions within a single device.
Implementation Method 1
the heater is configured to heat the detection chip
Implementation Method 2
the cooler is configured to cool the detection chip
Implementation Method 3
the optical sensor is configured to receive light from the detection chip and perform detection according to the light
Data Source
AI summary
The present disclosure provides an analysis method for a detection chip, an analysis device and an apparatus. and a method of operating the analysis device. The analysis method includes: illuminating the detection chip with light provided by a light source, to obtain light from the detection chip; acquiring an optical image of the detection chip by an image sensor; converting the optical image to a grayscale image; determining a spaced line in the grayscale image; dividing the grayscale image according to the spaced line to obtain a plurality of image blocks, and determining an image block with a pixel mean square error greater than a preset threshold in the plurality of image blocks as a target image block.