Recombinant Polypeptide DPV3-Hsp40-J for Cell Transduction
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Solution Overview
Problem
Current methods for enhancing cell transduction efficiency of therapeutic agents, such as proteins and nucleic acids, are not sufficient, as they often rely on liposome delivery which may not be effective for all types of agents.
Innovation Solution
Conjugating a cell penetrating peptide DPV3 with an Hsp40-J domain to create a recombinant polypeptide that enhances the cell transduction efficiency of target agents, including proteins, nucleic acids, and vaccines, by facilitating their entry into cells.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If liposome delivery method is used, then delivery system is established, but cell transduction efficiency is insufficient
Solution Approach 1:
The patent uses a recombinant polypeptide comprising a cell-penetrating peptide (CPP) and an Hsp40-J domain as an intermediary carrier to deliver therapeutic agents into cells. The CPP facilitates cell membrane penetration while the Hsp40-J domain enhances protein folding and cellular uptake, significantly improving transduction efficiency compared to conventional liposome delivery methods.
Solution Approach 2:
The invention creates a composite recombinant polypeptide structure combining the cell-penetrating peptide domain with the Hsp40-J domain. This composite molecular structure leverages the cell-penetration capability of CPP and the chaperone-like protein folding assistance of Hsp40-J to achieve enhanced cellular internalization and transduction of therapeutic agents.
2Productivity
If recombinant polypeptide with DPV3 and Hsp40-J domain is used, then cell transduction efficiency is enhanced, but protein expression system complexity increases
Solution Approach 1:
The recombinant polypeptide is segmented into two functional domains: a cell-penetrating peptide (CPP) segment that mediates membrane crossing and an Hsp40-J domain segment that facilitates protein folding and cellular processing. This segmentation allows each domain to perform its specific function independently, simplifying the overall design while maintaining high transduction efficiency.
Solution Approach 2:
The recombinant polypeptide structure serves multiple functions simultaneously: the CPP domain enables cell membrane penetration, the Hsp40-J domain assists in protein folding and stabilization, and together they facilitate efficient cellular internalization and transduction. This multi-functionality in a single molecular construct reduces the need for multiple separate delivery components.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The recombinant polypeptide significantly improves the cell transduction efficiency of therapeutic agents, often surpassing the effectiveness of liposome delivery methods, allowing for more efficient delivery and expression of recombinant proteins.
Implementation Method 1
X represents a cell penetrating peptide DPV3
Implementation Method 2
Y represents an Hsp40-J domain
Data Source
AI summary
The invention provides a recombinant polypeptide X—Y for enhancing cell transduction efficiency of a target agent, wherein X is a cell penetrating peptide DPV3, and Y is an Hsp40-J domain. Also provided is a method for enhancing cell transduction efficiency of a target agent, comprising conjugating/attaching said target agent with a recombinant polypeptide X—Y, wherein X is a cell penetrating peptide DPV3, and Y is an Hsp40-J domain. Further provided is a pharmaceutical composition comprising a therapeutic agent, wherein said therapeutic agent is modified by conjugating/attaching with a recombinant polypeptide X—Y, wherein X is a cell penetrating peptide DPV3, and Y is an Hsp40-J domain.