Dried Dye Reagent Devices for Analyte Detection
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Solution Overview
Problem
Existing assays for determining analyte presence and concentration in biological samples face challenges due to undesirable dye-dye interactions, which can quench fluorescence, leading to reduced detection accuracy and efficiency.
Innovation Solution
Dried dye reagent devices with dyes stably associated with high surface area solid supports minimize dye-dye interactions by maintaining dyes in a stable, non-interfering environment, allowing for precise and accurate analyte detection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If dyes are used in liquid form for analyte detection, then detection capability is achieved, but fluorescence quenching occurs due to dye-dye interactions
Solution Approach 1:
The patent changes the physical state of the dye from liquid to dried form, and alters the molecular environment by associating dyes with high surface area solid supports. This parameter change prevents dye-dye interactions that cause fluorescence quenching, thereby maintaining detection accuracy while eliminating the harmful effect of quenching in liquid solutions
Solution Approach 2:
The high surface area solid support acts as an intermediary between the dye molecules and the surrounding environment. This intermediary prevents direct dye-dye interactions by providing a stable association surface, thereby eliminating fluorescence quenching while preserving the dye's detection capability
2Stability of the object's composition
If dyes are stabilized in solution, then detection consistency is improved, but dye-dye interactions increase causing quenching
Solution Approach 1:
The patent changes the stabilization mechanism from solution-based to solid-support-based stabilization. By associating dyes with high surface area solid supports in a dried state, the dye molecules achieve stability without the harmful dye-dye interactions that occur in liquid solutions
Solution Approach 2:
The patent creates a localized environment for each dye molecule on the high surface area solid support, where dyes are stably associated but spatially separated. This local quality approach maintains dye stability while preventing the widespread dye-dye interactions that cause fluorescence quenching in bulk solutions
3Adaptability or versatility
If multiple dyes are used for multiplex detection, then analyte detection capability increases, but dye-dye interactions intensify causing quenching
Solution Approach 1:
The high surface area solid support serves as an intermediary that enables multiple dyes to coexist without harmful interactions. Each dye molecule is stably associated with the support surface, which prevents direct dye-dye interactions even when multiple different dyes are present for multiplex detection
Solution Approach 2:
The patent changes the physical and molecular environment by using dried dyes on solid supports, which fundamentally alters how multiple dyes interact (or don't interact). This parameter change enables multiplex detection capability while eliminating the quenching effect that would normally intensify with multiple dyes in solution
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution enhances the stability and detection efficiency of dyes in assays, reducing fluorescence quenching and maintaining accurate analyte detection over extended periods.
Implementation Method 1
one or more dyes stably associated with a high surface area solid support
Data Source
AI summary
Dried dye reagent devices are provided. Aspects of the devices include a container having positioned therein one or more dried dye compositions that include one or more dyes stably associated with a high surface area solid support. Aspects of the invention further include methods of making and using the devices, e.g., in analyte detection applications, as well as kits containing the devices.