Dried Oligonucleotide Composition Adhesion and Visual Detection

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Solution Overview

Problem

Dried oligonucleotides are prone to separation and loss during drying, storage, and transportation due to their colorless and transparent nature, leading to insufficient amounts for PCR reactions and resulting in incorrect experimental results.

Innovation Solution

Incorporating adhesive substances such as proteins, water-soluble polymers, nonionic surfactants, oligosaccharides, or polyalcohols into the oligonucleotide composition to prevent detachment from storage containers, along with the addition of non-reactive dyes for visual confirmation.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Quantity of substance

If oligonucleotide is aliquoted into storage container in trace amount, then it can be packaged for commercial sale, but it cannot be visually confirmed during drying or transporting

Engineering Contradiction:

Improveoligonucleotide amountVSAvoidvisual confirmation difficulty

Core Design Contradiction:

Quantity of substanceVSDifficulty of detecting and measuring

Solution Approach 1:

The patent applies color change principle by adding non-reactive dyes to the oligonucleotide composition. The dyes provide visual color indication that allows confirmation of oligonucleotide presence in the storage container during drying and transporting, while the dyes are specifically selected to be non-reactive with the oligonucleotide and not interfere with PCR reactions.

Inventive Principle:

Principle #32Color changes

2Duration of action of stationary object

If oligonucleotide is dried in storage container, then it can be stored and transported, but it may separate and adhere to lid causing loss

Engineering Contradiction:

Improvestorage stabilityVSAvoidoligonucleotide loss

Core Design Contradiction:

Duration of action of stationary objectVSLoss of substance

Solution Approach 1:

The patent applies intermediary principle by introducing adhesive substances as mediators between the oligonucleotide and the storage container. These adhesive substances prevent the oligonucleotide from adhering to the lid or separating during drying and storage, ensuring the oligonucleotide remains in the container without loss.

Inventive Principle:

Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent combines color change principle with the adhesive substance by adding non-reactive dyes that provide visual confirmation of oligonucleotide presence. This allows detection of any separation or loss events while the adhesive substance prevents them, creating a dual-function system that both protects and monitors the oligonucleotide.

Inventive Principle:

Principle #32Color changes

3Reliability

If adhesive substances are added to prevent oligonucleotide separation, then oligonucleotide loss is prevented, but composition complexity increases

Engineering Contradiction:

Improveoligonucleotide retentionVSAvoidcomposition complexity

Core Design Contradiction:

ReliabilityVSDevice complexity

Solution Approach 1:

The patent applies parameter changes principle by carefully controlling the concentration ranges of adhesive substances and non-reactive dyes. The adhesive substance is present at 0.01-5% w/v and non-reactive dye at 0.001-0.1% w/v, optimizing the balance between preventing oligonucleotide loss and maintaining composition simplicity. These controlled parameters ensure reliability while minimizing complexity.

Inventive Principle:

Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Prevents oligonucleotide loss during drying and storage, allowing for easy visual confirmation and reducing the failure rate of PCR experiments by ensuring accurate primer amounts.

Implementation Method 1

a substance for preventing the oligonucleotide from being separated and lost, which is adhesive to a storage container containing the oligonucleotide composition

Methodology Applied

Scientific EffectAdhesion: Adhesive

Implementation Method 2

along with the addition of non-reactive dyes for visual confirmation

Methodology Applied

Scientific EffectOptical detection:

Data Source

PatentUS9200027B2Dried oligonucleotide composition and method of producing the same

Publication Date: 2015.12.01 BIONEER

AI summary

The present invention relates to a dried oligonucleotide composition and a method for producing the same. More specifically, it relates to a dried oligonucleotide composition produced by the steps comprising adding a substance for preventing the oligonucleotide from being separated and lost, which is adhesive to a storage container containing the oligonucleotide composition, in order to prevent the oligonucleotide from being separated and lost during manufacturing and distributing the dried oligonucleotide composition, optionally adding a non-reactive dye substance, and drying the resulting solution. The dried oligonucleotide composition of the present invention can be prevented from being separated and lost during manufacturing step, or transporting step after packaging, and the presence or absence of the oligonucleotide in the storage container can be easily confirmed with naked eyes. Accordingly, unnecessary labor waste and time waste caused by the separation of the oligonucleotide upon experiment can be overcome.