Dried Reagent Strainers for Fluorescence Quenching
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Solution Overview
Problem
Existing assays for determining analyte presence and concentration in biological samples face challenges due to undesirable dye-dye interactions, which can quench fluorescence, leading to reduced detection accuracy.
Innovation Solution
Dried reagent strainers with filters containing multiple dried reagent compositions, including dyes, that are stably associated with the filter to minimize dye-dye interactions, allowing for precise and accurate analyte detection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If multiple dyes are used in liquid form for analyte detection, then detection capability is enhanced, but fluorescence quenching occurs due to dye-dye interactions
Solution Approach 1:
The patent changes the physical state of the dyes from liquid to dried form, and positions them on a solid filter support. This parameter change separates the dyes spatially and prevents their harmful interactions while maintaining their detection functions. The dried reagent compositions are applied to the filter in a controlled manner to ensure proper positioning and spacing.
2Stability of the object's composition
If dyes are stored in liquid solution, then they remain soluble and active, but they exhibit undesirable interactions that quench fluorescence
Solution Approach 1:
The patent extracts the dyes from their liquid solution state and deposits them as dried reagent compositions onto a solid filter support. This extraction removes the harmful liquid-phase interactions between dyes while preserving their analytical functions. The dyes are applied in a controlled drying process that maintains their stability and activity.
3Reliability
If dried reagent compositions are applied to the filter, then dye-dye interactions are minimized, but additional manufacturing steps are required
Solution Approach 1:
The patent merges the filter support function with the reagent delivery function into a single integrated component. The dried reagent compositions are applied directly to the filter surface, combining the filtration and analytical reagent delivery functions. This integration simplifies the overall device architecture and reduces the number of separate components needed.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The use of dried reagent strainers with distinct dye compositions on the filter reduces fluorescence quenching, maintaining high detection accuracy and stability over extended periods, facilitating efficient analyte analysis in biological samples.
Implementation Method 1
a body having an opening with a filter positioned in the opening, where the filter includes a dried reagent composition
Implementation Method 2
dried reagent compositions that are stably associated with the filter
Implementation Method 3
The detectable label may be a marker that can be visualized either by an unaided eye or detectable by spectroscopy, such as fluorescence or UV-vis spectroscopy
Implementation Method 4
undesirable dye-dye interaction where the fluorescence of a dye is significantly less than would be expected as compared to the dye's fluorescence in the absence of other interfering dyes
Data Source
AI summary
Dried reagent strainers, such as cell strainers are provided. Aspects of the strainers include a body having an opening with a filter positioned in the opening, where the filter includes a dried reagent composition. Aspects of the invention further include methods of making and using the strainers, as well as kits containing the strainers.