Droplet Analysis Image Correction for Optical Signal Uniformity
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for analyzing droplets, such as plane scanning, face challenges in accurately determining droplet volume and state due to non-uniform optical signal intensity caused by varying droplet volumes and low spatial stability, leading to calculation errors and issues like droplet displacement.
Innovation Solution
A method involving the acquisition and processing of working dye and reference dye channel images, including image correction, enhancement, and noise reduction, followed by registration and region determination to accurately analyze droplet systems by calculating relative light intensities.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If the method of plane scanning is used to capture images of droplets, then the throughput is improved by obtaining image information of all droplets at one time, but the measurement precision deteriorates due to non-uniform optical signal intensity caused by varying droplet volumes
Solution Approach 1:
The patent applies local quality by performing individualized processing on each droplet's optical signal. Instead of treating all droplets uniformly, the system calculates correction coefficients for each droplet based on its specific volume and optical path length, then applies localized correction to each droplet's signal. This ensures that each droplet's measurement is adjusted according to its unique characteristics, resolving the precision issue while maintaining high throughput.
Solution Approach 2:
The patent changes the parameter of optical signal intensity by introducing correction coefficients that account for droplet volume variations. The system transforms the raw optical signals into corrected signals by applying these coefficients, effectively normalizing the measurements across droplets of different volumes. This parameter transformation allows accurate comparison and analysis while preserving the high-speed imaging capability.
2Productivity
If the method of plane scanning is used to capture images of droplets, then the throughput is improved by obtaining image information of all droplets at one time, but the reliability deteriorates due to droplet displacement and repeated counting caused by low spatial stability
Solution Approach 1:
The patent applies preliminary action by performing image registration and droplet positioning before final analysis. The system captures reference images to establish a spatial map of droplet positions, then uses this information to correct and align subsequent images. This preliminary spatial calibration prevents repeated counting and ensures reliable identification of each droplet, maintaining both high throughput and accuracy.
3Adaptability or versatility
If droplet volume changes or the droplet system no longer has a single volume, then the adaptability is improved by accommodating multi-volume droplets, but the measurement precision deteriorates due to height changes affecting uniformity of optical signals
Solution Approach 1:
The patent applies local quality by calculating and applying individual correction coefficients for each droplet based on its specific volume and height. Instead of requiring uniform droplet volumes, the system adapts to each droplet's unique geometry by locally adjusting the optical signal correction. This approach enables accurate measurement of multi-volume droplet systems while maintaining measurement precision through droplet-specific parameter optimization.
Data Source
AI summary
A method for analyzing droplets based on images: obtaining a working dye channel image and a reference dye channel image corresponding to a droplet system (S1); separately performing a preset image processing operation on the working dye channel image and the reference dye channel image (S2); using the working dye channel image or the reference dye channel image as a target image (S3), and determining a region in which each droplet is located from within the target image (S4); and on the basis of the determined region in which each droplet is located, analyzing the droplet system (S5). The method can effectively improve the accuracy of droplet analysis. Further provided are a computer device and a storage medium.