Dry Platelet Composition Freeze-Drying Cryoprotectant Inhibitors
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Solution Overview
Problem
Current methods for preserving platelets are limited by their short shelf-life and inability to maintain normal platelet function after storage, restricting their availability for therapeutic use.
Innovation Solution
Development of a dry platelet composition involving freeze-dried platelets rehydrated with a cryopreservative additive containing inhibitors of platelet activation and cryoprotectants, which retains platelet function and extends shelf-life.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Duration of action of stationary object
If platelets are stored using current preservation methods, then platelet availability is maintained, but shelf-life is limited and normal platelet function is lost after storage
Solution Approach 1:
The patent applies parameter changes by transitioning platelets from liquid to dry frozen state, altering physical conditions to extend shelf-life while maintaining function. Specifically, platelets are frozen in a dry state with controlled concentrations of cryoprotectants and activation inhibitors, changing the storage parameters to achieve both extended duration and preserved functionality
Solution Approach 2:
The patent uses intermediaries including cryoprotectants (such as DMSO, glycerol, or sugars) and platelet activation inhibitors (such as adenosine, aspirin, or prostaglandins) that mediate between the storage conditions and platelet viability. These intermediaries protect platelets during freezing and storage while preventing unwanted activation, thereby maintaining function throughout extended storage periods
2Duration of action of stationary object
If platelets are dried and rehydrated without inhibitors of platelet activation, then shelf-life is extended, but platelet activation and function are compromised
Solution Approach 1:
The patent applies preliminary anti-action by incorporating platelet activation inhibitors into the freezing mixture before drying. These inhibitors (such as adenosine, aspirin, or other anti-platelet agents) are present during the freezing and storage process to preemptively prevent platelet activation, thereby maintaining platelet functionality after rehydration and extending usable shelf-life
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The dry platelet composition maintains substantial platelet function and viability upon rehydration, enhancing their utility in treating wounds and platelet-related disorders by extending their shelf-life and functionality.
Implementation Method 1
The invention features dry platelet compositions and methods of making a freeze-dried platelet composition
Implementation Method 2
The invention is based, in part, on the discovery that platelets dried and rehydrated in the presence of a cryopreservative additive
Data Source
AI summary
The invention features a dry platelet composition and methods of making and using the freeze-dried platelet composition.