Dry Reagent Compositions for Intracellular Bacterial Detection
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Solution Overview
Problem
Current methods for detecting intracellular bacterial enzymes require multiple steps and solutions, making them time-consuming and inefficient.
Innovation Solution
Solid supports and dry compositions containing a lysis reagent and an intracellular microorganism target detection reagent in a dried form, which combine the necessary reagents for lysing microorganisms and detecting intracellular targets in a single step, eliminating the need for multiple preparation steps and maintaining stability at room temperature.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Loss of time
If multiple steps and solutions are used for detecting intracellular bacterial enzymes, then detection accuracy can be maintained, but the process becomes time-consuming and inefficient
Solution Approach 1:
The patent combines multiple separate reagents (lysis buffer and detection reagent) into a single integrated dry composition that contains both components in predetermined amounts. This merging eliminates the need for separate preparation steps and reduces detection time while maintaining detection accuracy through optimized reagent combinations.
Solution Approach 2:
The patent performs preliminary action by pre-drying and pre-formulating the reagents into a stable dry composition before use. This preliminary preparation allows the reagents to be stored stably and eliminates the need for on-site preparation of multiple solutions, directly reducing the time and complexity of the detection process.
2Stability of the object's composition
If reagents are kept in dried form, then stability at room temperature is improved, but the complexity of handling and reconstitution may increase
Solution Approach 1:
The patent applies parameter changes by transitioning reagents from liquid to dry form, which fundamentally changes their storage and handling parameters. This parameter change enables room temperature stability while the formulation includes all necessary components pre-measured, simplifying the reconstitution step to a single mixing action.
3Productivity
If a one-step process is implemented, then productivity increases, but the requirement for precise reagent formulation and stabilization becomes more critical
Solution Approach 1:
The patent performs preliminary action by pre-formulating and pre-drying the reagents with precise measurements during manufacturing. This preliminary precision work is embedded into the dry composition, allowing the final one-step detection process to achieve high productivity without requiring precision during the actual detection operation.
Data Source
AI summary
Presented herein are solid supports on or in which a composition comprising a lysis reagent and an intracellular microorganism target detection reagent (e.g., an intracellular bacterial target detection reagent) is present in a dried form. Presented herein are also methods for detecting the presence of an intracellular microorganism target (e.g., an intracellular bacterial target) utilizing such solid supports. Further, presented herein are tablets as well as dry powders comprising a lysis reagent and an intracellular microorganism target detection reagent (e.g., an intracellular bacterial target detection reagent), and methods of using such tablets and dry powders to detect the presence of an intracellular microorganism target (e.g., an intracellular bacterial target).